Cloning and analysis of cDNA encoding murine pinin

Pin Ouyang*, Yen Yi Zhen, Stephen P. Sugrue

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

11 Scopus citations

Abstract

Pinin is a cell junction-associated protein involved in the stabilization of the desmosome-intermediate filament complex in various epithelial tissues. Utilizing a cDNA probe derived from canine pinin, we isolated overlapping cDNA clones encoding murine full-length pinin. The total cDNA contained an open reading frame of 2175 nucleotides coding for 725 amino acids as well as a 3'- and a 5'-untranslated regions of 620 and 18 nucleotides, respectively. The overall predicted amino acid sequence of mouse pinin displayed strong identities to those of canine and human pinin, with the exception of a stretch of 38 amino acids which were found to be deleted in mouse pinin. There were several discernible domains found within mouse pinin. These included three coiled-coil domains, a small stretch of glycine loops, a short glutamine-proline-rich domain and a polyserine domain.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalGene
Volume197
Issue number1-2
DOIs
StatePublished - 15 09 1997

Keywords

  • Desmosome
  • Molecular cloning
  • Pinin

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