Cloning and preliminary characterization of srf-2020 and srf-801, the recF partial suppressor mutations which map in recA of Escherichia coli K-12

T. C.V. Wang*, M. V.V.S. Madiraju, A. Templin, A. J. Clark

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations

Abstract

We have located the single nucleotide changes suffered in recA sequence2 recF partial suppresor mutations: srf-2020 at codon 121 and srf-801 at codon 257. srf-2020 changes codon 121 from threonine (ACC) to isoleucine (ATC). srf-801 changes codon 257 from glutamine (CAG) to proline (CCG). Consequently these mutations were renamed recA2020 and recA801 respectively. Preliminary characterization of recA2020 revealed that it is transdominant to recA+, like recA803, another recF partial suppressor. Interactions of recA2020 with recA803 were examined using genetic studies. Heterozygotes containing recA2020 and recA803 failed to produce a synergistic suppression effect in suppressing the recF deficiency. Presence of both recA2020 and recA803 mutations in the same recA gene also failed to produce any greater amount of UV resistance to a uvrA6recF143del(recA) strain indicating no interactions between these suppressors.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalBiochimie
Volume73
Issue number2-3
DOIs
StatePublished - 1991

Keywords

  • DNA cloning
  • DNA repair
  • DNA sequencing
  • recA mutants
  • recF suppressors

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