CNTs gated nanofluidic system for single bacterium detection by GNPs-based redox signal amplification

Po Chao Wen, Jen Kuei Wu, Hwan You Chang, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this work, we report a portable microfluidic chip based on gold nanoparticle-enhanced electrochemical detection for single-bacteria diognostic. The possibility of single-bacteria detection is realized here by using electroactive molecules modified on gold nanoparticles (NPs) to detect and differentiate Staphylococcus aureus and Pseudomonas aeruginosa. The detection limit of these two electroactive molecules is near femtomolar (1 pM) when immobilized on gold nanoparticles (NPs), and the minima amounts of gold NPs required in the detection is around thousands (103 particle/μl). A concentration of couples whole bacteria (<10 cells/μl) is detected by CV measurements. The liner detection limit concention is form 1-1000 cells/μl. The results showed that the portable biochip system has great potential as a device for single-particle or possibly even single-organism detection.

Original languageEnglish
Title of host publication8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages290-293
Number of pages4
DOIs
StatePublished - 2013
Externally publishedYes
Event8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
Duration: 07 04 201310 04 2013

Publication series

Name8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013

Conference

Conference8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Country/TerritoryChina
CitySuzhou
Period07/04/1310/04/13

Keywords

  • gold nanoparticles
  • redox signal amplification
  • single bacteria detection

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