Co-activation of Nonselective Cation Channels by Store Depletion and Oxidative Stress in Monocytic U937 Cells

Hui Fang Li, Ai Yu Shen, Chung Ren Jan, Sheng Nan Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

9 Scopus citations


Two different types of nonselective cation currents in monocytic U937 cells were characterized with the aid of patch-clamp technique. When the cells were exposed to oxidative stress with 10 mM H 2 O 2 , a nonselective cation current (I NS ) can be elicited. This current was still observed when extracellular cations are Ca 2+ . The change in intracellular Cl - concentrations did not shift the reversal potential of this current. This current showed no any rectification, and was time- and voltage-independent at any potentials. The further addition of LaCl 3 (100 μM) or dithiothreitol (10 μM) effectively suppressed this current However, SK & F 96365 (100 μM) or nifedipine (10 μM) did not produce any effect on it significantly. On the other hand, depletion of Ca 2+ stores by different maneuvers, such as the application of ATP, thapsigargin or A23187, or dialysis of the cells with inositol-1,4,5-trisphosphate activated another type of nonselective current which was identified as store-operated Ca 2+ -permeable current (I SOC )- Unlike H 2 O 2 -induced I NS , this current was observed to slowly inactivate when the voltage steps were hyperpolarized. Current-voltage relation of this current showed inward rectification. When the cell was challenged with both store depletion and H 2 O 2 , I NS on the top of I SOC can be activated. These results suggest that different types of nonselective cation channels can be co-expressed in the same cell. Therefore, in U937 cells nonselective cation currents can be activated after the stimulation with oxidative stress, store depletion or both. The activation of these nonseiective cation channels may affect intracellular Ca 2+ homeostasis.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalChinese Journal of Physiology
Issue number2
StatePublished - 30 06 1998
Externally publishedYes


  • Monocyte
  • Nonselective cation current
  • Oxidative stress


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