Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube

Ashleigh J. Crawford; André Forjaz; Joanna Bons; Isha Bhorkar; Triya Roy; David Schell; Vasco Queiroga; Kehan Ren; Donald Kramer; Wilson Huang; Gabriella C. Russo; Meng Horng Lee; Pei Hsun Wu; Ie Ming Shih; Tian Li Wang; Mark A. Atkinson; Birgit Schilling; Ashley L. Kiemen; Denis Wirtz

doi:10.1126/sciadv.adp6285

Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube

Ashleigh J. Crawford, André Forjaz, Joanna Bons, Isha Bhorkar, Triya Roy, David Schell, Vasco Queiroga, Kehan Ren, Donald Kramer, Wilson Huang, Gabriella C. Russo, Meng Horng Lee, Pei Hsun Wu, Ie Ming Shih, Tian Li Wang, Mark A. Atkinson, Birgit Schilling, Ashley L. Kiemen, Denis Wirtz^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

4 Scopus citations

Abstract

The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.

Original language	English
Article number	eadp6285
Pages (from-to)	eadp6285
Journal	Science Advances
Volume	10
Issue number	39
DOIs	https://doi.org/10.1126/sciadv.adp6285
State	Published - 27 09 2024

Bibliographical note

Publisher Copyright:
© 2024 the Authors, some rights reserved.

Keywords

Humans
Female
Fallopian Tubes/anatomy & histology
Imaging, Three-Dimensional/methods
Proteomics/methods
Models, Biological
Single-Cell Analysis/methods

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1126/sciadv.adp6285

Cite this

Crawford, A. J., Forjaz, A., Bons, J., Bhorkar, I., Roy, T., Schell, D., Queiroga, V., Ren, K., Kramer, D., Huang, W., Russo, G. C., Lee, M. H., Wu, P. H., Shih, I. M., Wang, T. L., Atkinson, M. A., Schilling, B., Kiemen, A. L., & Wirtz, D. (2024). Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube. Science Advances, 10(39), eadp6285. Article eadp6285. https://doi.org/10.1126/sciadv.adp6285

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title = "Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube",

abstract = "The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.",

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author = "Crawford, {Ashleigh J.} and Andr{\'e} Forjaz and Joanna Bons and Isha Bhorkar and Triya Roy and David Schell and Vasco Queiroga and Kehan Ren and Donald Kramer and Wilson Huang and Russo, {Gabriella C.} and Lee, {Meng Horng} and Wu, {Pei Hsun} and Shih, {Ie Ming} and Wang, {Tian Li} and Atkinson, {Mark A.} and Birgit Schilling and Kiemen, {Ashley L.} and Denis Wirtz",

note = "Publisher Copyright: {\textcopyright} 2024 the Authors, some rights reserved.",

year = "2024",

month = sep,

day = "27",

doi = "10.1126/sciadv.adp6285",

language = "英语",

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Crawford, AJ, Forjaz, A, Bons, J, Bhorkar, I, Roy, T, Schell, D, Queiroga, V, Ren, K, Kramer, D, Huang, W, Russo, GC, Lee, MH, Wu, PH, Shih, IM, Wang, TL, Atkinson, MA, Schilling, B, Kiemen, AL & Wirtz, D 2024, 'Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube', Science Advances, vol. 10, no. 39, eadp6285, pp. eadp6285. https://doi.org/10.1126/sciadv.adp6285

TY - JOUR

T1 - Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube

AU - Crawford, Ashleigh J.

AU - Forjaz, André

AU - Bons, Joanna

AU - Bhorkar, Isha

AU - Roy, Triya

AU - Schell, David

AU - Queiroga, Vasco

AU - Ren, Kehan

AU - Kramer, Donald

AU - Huang, Wilson

AU - Russo, Gabriella C.

AU - Lee, Meng Horng

AU - Wu, Pei Hsun

AU - Shih, Ie Ming

AU - Wang, Tian Li

AU - Atkinson, Mark A.

AU - Schilling, Birgit

AU - Kiemen, Ashley L.

AU - Wirtz, Denis

PY - 2024/9/27

Y1 - 2024/9/27

N2 - The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.

AB - The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.

KW - Humans

KW - Female

KW - Fallopian Tubes/anatomy & histology

KW - Imaging, Three-Dimensional/methods

KW - Proteomics/methods

KW - Models, Biological

KW - Single-Cell Analysis/methods

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U2 - 10.1126/sciadv.adp6285

DO - 10.1126/sciadv.adp6285

M3 - 文章

C2 - 39331707

AN - SCOPUS:85205276210

SN - 2375-2548

VL - 10

SP - eadp6285

JO - Science Advances

JF - Science Advances

IS - 39

M1 - eadp6285

ER -

Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube

Abstract

Bibliographical note

Keywords

UN SDGs

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