Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle

Tsung Cheng Yin; Re Wen Wu; Jiunn Jye Sheu; Pei Hsun Sung; Kuan Hung Chen; John Y. Chiang; Shu Kai Hsueh; Wen Jung Chung; Pao Yuan Lin; Shan Ling Hsu; Chien Chang Chen; Chen Yu Chen; Pei Lin Shao; Hon Kan Yip

doi:10.1155/2018/6012636

Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle

Tsung Cheng Yin, Re Wen Wu, Jiunn Jye Sheu, Pei Hsun Sung, Kuan Hung Chen, John Y. Chiang, Shu Kai Hsueh, Wen Jung Chung, Pao Yuan Lin, Shan Ling Hsu, Chien Chang Chen, Chen Yu Chen, Pei Lin Shao, Hon Kan Yip^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Journal Article › peer-review

23 Scopus citations

Abstract

Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats (n = 30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm² given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 10⁶ cell intramuscular and 1.2 × 10⁶ cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p < 0 0001). By day 5 after IR, the mechanical paw-withdrawal threshold displayed an opposite pattern of CPK (all p < 0 0001). The protein expressions of inflammatory, oxidative-stress, apoptotic, fibrotic, DNA-damaged, and mitochondrial-damaged biomarkers and cellular expressions of inflammatory and DNA-damaged biomarkers exhibited an identical pattern of CPK among the five groups (all p < 0 0001). The microscopic findings of endothelial-cell biomarkers and number of arterioles expressed an opposite pattern of CPK, and the angiogenesis marker was significantly progressively increased from groups 1 to 5, whereas the histopathology showed that muscle-damaged/fibrosis/collagen-deposition areas exhibited an identical pattern of CPK among the five groups (all p < 0 0001). In conclusion, ECSW-ADMSC therapy is superior to either one applied individually for protecting against IR-induced thigh injury.

Original language	English
Article number	6012636
Journal	Oxidative Medicine and Cellular Longevity
Volume	2018
DOIs	https://doi.org/10.1155/2018/6012636
State	Published - 2018

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Publisher Copyright:
Copyright © 2018 Tsung-Cheng Yin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yin, T. C., Wu, R. W., Sheu, J. J., Sung, P. H., Chen, K. H., Chiang, J. Y., Hsueh, S. K., Chung, W. J., Lin, P. Y., Hsu, S. L., Chen, C. C., Chen, C. Y., Shao, P. L., & Yip, H. K. (2018). Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle. Oxidative Medicine and Cellular Longevity, 2018, Article 6012636. https://doi.org/10.1155/2018/6012636

@article{741b150126be495599870936e73bf324,

title = "Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle",

abstract = "Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats (n = 30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm2 given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 106 cell intramuscular and 1.2 × 106 cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p < 0 0001). By day 5 after IR, the mechanical paw-withdrawal threshold displayed an opposite pattern of CPK (all p < 0 0001). The protein expressions of inflammatory, oxidative-stress, apoptotic, fibrotic, DNA-damaged, and mitochondrial-damaged biomarkers and cellular expressions of inflammatory and DNA-damaged biomarkers exhibited an identical pattern of CPK among the five groups (all p < 0 0001). The microscopic findings of endothelial-cell biomarkers and number of arterioles expressed an opposite pattern of CPK, and the angiogenesis marker was significantly progressively increased from groups 1 to 5, whereas the histopathology showed that muscle-damaged/fibrosis/collagen-deposition areas exhibited an identical pattern of CPK among the five groups (all p < 0 0001). In conclusion, ECSW-ADMSC therapy is superior to either one applied individually for protecting against IR-induced thigh injury.",

author = "Yin, {Tsung Cheng} and Wu, {Re Wen} and Sheu, {Jiunn Jye} and Sung, {Pei Hsun} and Chen, {Kuan Hung} and Chiang, {John Y.} and Hsueh, {Shu Kai} and Chung, {Wen Jung} and Lin, {Pao Yuan} and Hsu, {Shan Ling} and Chen, {Chien Chang} and Chen, {Chen Yu} and Shao, {Pei Lin} and Yip, {Hon Kan}",

note = "Publisher Copyright: Copyright {\textcopyright} 2018 Tsung-Cheng Yin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",

year = "2018",

doi = "10.1155/2018/6012636",

language = "英语",

volume = "2018",

journal = "Oxidative Medicine and Cellular Longevity",

issn = "1942-0900",

publisher = "Hindawi Publishing Corporation",

}

Yin, TC, Wu, RW, Sheu, JJ, Sung, PH, Chen, KH, Chiang, JY, Hsueh, SK, Chung, WJ, Lin, PY, Hsu, SL, Chen, CC, Chen, CY, Shao, PL & Yip, HK 2018, 'Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle', Oxidative Medicine and Cellular Longevity, vol. 2018, 6012636. https://doi.org/10.1155/2018/6012636

Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle. / Yin, Tsung Cheng; Wu, Re Wen; Sheu, Jiunn Jye et al.
In: Oxidative Medicine and Cellular Longevity, Vol. 2018, 6012636, 2018.

Research output: Contribution to journal › Journal Article › peer-review

TY - JOUR

T1 - Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle

AU - Yin, Tsung Cheng

AU - Wu, Re Wen

AU - Sheu, Jiunn Jye

AU - Sung, Pei Hsun

AU - Chen, Kuan Hung

AU - Chiang, John Y.

AU - Hsueh, Shu Kai

AU - Chung, Wen Jung

AU - Lin, Pao Yuan

AU - Hsu, Shan Ling

AU - Chen, Chien Chang

AU - Chen, Chen Yu

AU - Shao, Pei Lin

AU - Yip, Hon Kan

N1 - Publisher Copyright: Copyright © 2018 Tsung-Cheng Yin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2018

Y1 - 2018

N2 - Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats (n = 30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm2 given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 106 cell intramuscular and 1.2 × 106 cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p < 0 0001). By day 5 after IR, the mechanical paw-withdrawal threshold displayed an opposite pattern of CPK (all p < 0 0001). The protein expressions of inflammatory, oxidative-stress, apoptotic, fibrotic, DNA-damaged, and mitochondrial-damaged biomarkers and cellular expressions of inflammatory and DNA-damaged biomarkers exhibited an identical pattern of CPK among the five groups (all p < 0 0001). The microscopic findings of endothelial-cell biomarkers and number of arterioles expressed an opposite pattern of CPK, and the angiogenesis marker was significantly progressively increased from groups 1 to 5, whereas the histopathology showed that muscle-damaged/fibrosis/collagen-deposition areas exhibited an identical pattern of CPK among the five groups (all p < 0 0001). In conclusion, ECSW-ADMSC therapy is superior to either one applied individually for protecting against IR-induced thigh injury.

AB - Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats (n = 30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm2 given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 106 cell intramuscular and 1.2 × 106 cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p < 0 0001). By day 5 after IR, the mechanical paw-withdrawal threshold displayed an opposite pattern of CPK (all p < 0 0001). The protein expressions of inflammatory, oxidative-stress, apoptotic, fibrotic, DNA-damaged, and mitochondrial-damaged biomarkers and cellular expressions of inflammatory and DNA-damaged biomarkers exhibited an identical pattern of CPK among the five groups (all p < 0 0001). The microscopic findings of endothelial-cell biomarkers and number of arterioles expressed an opposite pattern of CPK, and the angiogenesis marker was significantly progressively increased from groups 1 to 5, whereas the histopathology showed that muscle-damaged/fibrosis/collagen-deposition areas exhibited an identical pattern of CPK among the five groups (all p < 0 0001). In conclusion, ECSW-ADMSC therapy is superior to either one applied individually for protecting against IR-induced thigh injury.

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U2 - 10.1155/2018/6012636

DO - 10.1155/2018/6012636

M3 - 文章

C2 - 29805730

AN - SCOPUS:85055073529

SN - 1942-0900

VL - 2018

JO - Oxidative Medicine and Cellular Longevity

JF - Oxidative Medicine and Cellular Longevity

M1 - 6012636

ER -

Combined therapy with extracorporeal shock wave and adipose-derived mesenchymal stem cells remarkably improved acute ischemia-reperfusion injury of quadriceps muscle

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