Comparison of Two Quantitative PCR–Based Assays for Detection of Minimal Residual Disease in B-Precursor Acute Lymphoblastic Leukemia Harboring Three Major Fusion Transcripts

Ying Jung Huang, Ming Chung Kuo, Tang Her Jaing, Hsi Che Liu, Ting Chi Yeh, Shih Hsiang Chen, Tung Liang Lin, Chao Ping Yang, Po Nan Wang, Jiunn Ming Sheen, Te Kau Chang, Chia Hui Chang, Shu Fen Hu, Ting Yu Huang, Shih Chung Wang, Kang Hsi Wu, Shyh Shin Chiou, Chih Cheng Hsiao, Lee Yung Shih*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Two quantitative PCR (qPCR)–based methods, for clonal immunoglobulin or T-cell receptor gene (Ig/TCR) rearrangements and for fusion transcripts, are widely used for the measurement of minimal residual disease (MRD) in patients with B-precursor acute lymphoblastic leukemia (ALL). MRD of bone marrow samples from 165 patients carrying the three major fusion transcripts, including 74 BCR-ABL1, 54 ETV6-RUNX1, and 37 TCF3-PBX1, was analyzed by using the two qPCR-based methods. The correlation coefficient of both methods was good for TCF3-PBX1 (R2 = 0.8088) and BCR-ABL1 (R2 = 0.8094) ALL and moderate for ETV6-RUNX1 (R2 = 0.5972). The concordance was perfect for TCF3-PBX1 ALL (97.2%), substantially concordant for ETV6-RUNX1 ALL (87.1%), and only moderate for BCR-ABL1 ALL (70.6%). The discordant MRD, positive for only one method with a difference greater than one log, was found in 4 of 93 samples (4.3%) with ETV6-RUNX1, 31 of 245 samples (12.7%) with BCR-ABL1, and none of TCF3-PBX1 ALL. None of the eight non-transplanted patients with BCR-ABL1-MRD (+)/Ig/TCR-MRD (–) with a median follow-up time of 73.5 months had hematologic relapses. Our study showed an excellent MRD concordance between the two qPCR-based methods in TCF3-PBX1 ALL, whereas qPCR for Ig/TCR is more reliable in BCR-ABL1 ALL.

Original languageEnglish
Pages (from-to)1373-1379
Number of pages7
JournalJournal of Molecular Diagnostics
Volume23
Issue number10
DOIs
StatePublished - 10 2021

Bibliographical note

Publisher Copyright:
© 2021 Association for Molecular Pathology and American Society for Investigative Pathology

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