Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer

Show Li Chen; Chih Ping Han; Yeou Ping Tsao

doi:10.1016/0166-0934(93)90179-U

Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer

Show Li Chen^*, Chih Ping Han, Yeou Ping Tsao

^*Corresponding author for this work

National Defense Medical Center Taiwan

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.

Original language	English
Pages (from-to)	89-94
Number of pages	6
Journal	Journal of Virological Methods
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/0166-0934(93)90179-U
State	Published - 04 1993
Externally published	Yes

Keywords

E5a protein
Human papillomavirus
Mutant primer
Polymerase chain reaction

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10.1016/0166-0934(93)90179-U

Cite this

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abstract = "The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.",

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AU - Han, Chih Ping

AU - Tsao, Yeou Ping

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AB - The method of Perrin and Gilliland (1990) was modified to create site-specific mutants. The polymerase chain reaction and a single mutant primer are needed to carry out site-specific mutagenesis. Using this method, removal of the excess primers and nucleotides from the initial amplification is not necessary. This method provides a simpler way to generate site-specific mutants.

KW - E5a protein

KW - Human papillomavirus

KW - Mutant primer

KW - Polymerase chain reaction

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Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primer

Abstract

Keywords

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