Continuous cell lysis devices using optically-induced electric field

Yen Heng Lin; Gwo Bin Lee

doi:10.1115/MNHMT2009-18333

Continuous cell lysis devices using optically-induced electric field

Yen Heng Lin, Gwo Bin Lee^*

^*Corresponding author for this work

Department of Biomedical Engineering

National Cheng Kung University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper presents a new integrated microfluidic device capable of counting and continuously lysing cells by using hydrodynamic forces and optically-induced electric field. First, the cells were focused in the central stream using hydrodynamic sheath flows. Then the focused cells passed through the buried optical fibers such that the number of cells can be counted optically. For 13-μm lung cancer cells, a total of 97 cells were counted without any missed. The counting accuracy can be as high as 100 %. After counting, cells were continuously disrupted using the optically-induced electric field. At an applied voltage of 20 Vpp with a frequency of 30 kHz, the lysis rate can be high as 100 % when the length of illuminated light was 150 μm,. The developed chip is therefore promising for intercellular constituent analysis and other cell-based studies.

Original language	English
Title of host publication	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Pages	169-172
Number of pages	4
DOIs	https://doi.org/10.1115/MNHMT2009-18333
State	Published - 2010
Event	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China Duration: 18 12 2009 → 21 12 2009

Publication series

Name	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Volume	1

Conference

Conference	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Country/Territory	China
City	Shanghai
Period	18/12/09 → 21/12/09

Keywords

Cell lysis
Flow cytometer
ODEP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1115/MNHMT2009-18333

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Lin, Y. H., & Lee, G. B. (2010). Continuous cell lysis devices using optically-induced electric field. In Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 (pp. 169-172). (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1). https://doi.org/10.1115/MNHMT2009-18333

@inproceedings{2f9d9ec3faf2474ca0337fdd90a88494,

title = "Continuous cell lysis devices using optically-induced electric field",

abstract = "This paper presents a new integrated microfluidic device capable of counting and continuously lysing cells by using hydrodynamic forces and optically-induced electric field. First, the cells were focused in the central stream using hydrodynamic sheath flows. Then the focused cells passed through the buried optical fibers such that the number of cells can be counted optically. For 13-μm lung cancer cells, a total of 97 cells were counted without any missed. The counting accuracy can be as high as 100 %. After counting, cells were continuously disrupted using the optically-induced electric field. At an applied voltage of 20 Vpp with a frequency of 30 kHz, the lysis rate can be high as 100 % when the length of illuminated light was 150 μm,. The developed chip is therefore promising for intercellular constituent analysis and other cell-based studies.",

keywords = "Cell lysis, Flow cytometer, ODEP",

author = "Lin, {Yen Heng} and Lee, {Gwo Bin}",

year = "2010",

doi = "10.1115/MNHMT2009-18333",

language = "英语",

isbn = "9780791843895",

series = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

pages = "169--172",

booktitle = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

note = "ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 ; Conference date: 18-12-2009 Through 21-12-2009",

}

Lin, YH & Lee, GB 2010, Continuous cell lysis devices using optically-induced electric field. in Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, vol. 1, pp. 169-172, ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, Shanghai, China, 18/12/09. https://doi.org/10.1115/MNHMT2009-18333

Continuous cell lysis devices using optically-induced electric field. / Lin, Yen Heng; Lee, Gwo Bin.
Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 169-172 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - This paper presents a new integrated microfluidic device capable of counting and continuously lysing cells by using hydrodynamic forces and optically-induced electric field. First, the cells were focused in the central stream using hydrodynamic sheath flows. Then the focused cells passed through the buried optical fibers such that the number of cells can be counted optically. For 13-μm lung cancer cells, a total of 97 cells were counted without any missed. The counting accuracy can be as high as 100 %. After counting, cells were continuously disrupted using the optically-induced electric field. At an applied voltage of 20 Vpp with a frequency of 30 kHz, the lysis rate can be high as 100 % when the length of illuminated light was 150 μm,. The developed chip is therefore promising for intercellular constituent analysis and other cell-based studies.

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KW - Flow cytometer

KW - ODEP

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Continuous cell lysis devices using optically-induced electric field

Abstract

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Keywords

UN SDGs

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