Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.

Chuck C.-K. Chao; WC Yam; S Lin-Chao

doi:10.1016/0006-291X(90)91411-K

Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.

Chuck C.-K. Chao, WC Yam, S Lin-Chao

Department of Biochemistry

Research output: Contribution to journal › Journal Article › peer-review

Abstract

The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.

Original language	American English
Pages (from-to)	431-438
Journal	Biochemical and Biophysical Research Communications
Volume	171
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91411-K
State	Published - 1990

Keywords

Base Sequence
Blotting, Northern
Calcimycin/pharmacology
Carrier Proteins/genetics
Cell Line
Gene Expression Regulation/drug effects
Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
Heat-Shock Proteins
Humans
Molecular Chaperones
Molecular Sequence Data
Promoter Regions, Genetic
RNA, Messenger/genetics
Regulatory Sequences, Nucleic Acid

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10.1016/0006-291X(90)91411-K

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title = "Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.",

abstract = "The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.",

keywords = "Base Sequence, Blotting, Northern, Calcimycin/pharmacology, Carrier Proteins/genetics, Cell Line, Gene Expression Regulation/drug effects, Glyceraldehyde-3-Phosphate Dehydrogenases/genetics, Heat-Shock Proteins, Humans, Molecular Chaperones, Molecular Sequence Data, Promoter Regions, Genetic, RNA, Messenger/genetics, Regulatory Sequences, Nucleic Acid",

author = "Chao, {Chuck C.-K.} and WC Yam and S Lin-Chao",

year = "1990",

doi = "10.1016/0006-291X(90)91411-K",

language = "American English",

volume = "171",

pages = "431--438",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.

AU - Chao, Chuck C.-K.

AU - Yam, WC

AU - Lin-Chao, S

PY - 1990

Y1 - 1990

N2 - The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.

AB - The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.

KW - Base Sequence

KW - Blotting, Northern

KW - Calcimycin/pharmacology

KW - Carrier Proteins/genetics

KW - Cell Line

KW - Gene Expression Regulation/drug effects

KW - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics

KW - Heat-Shock Proteins

KW - Humans

KW - Molecular Chaperones

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - RNA, Messenger/genetics

KW - Regulatory Sequences, Nucleic Acid

U2 - 10.1016/0006-291X(90)91411-K

DO - 10.1016/0006-291X(90)91411-K

M3 - Journal Article

C2 - 2118350

SN - 0006-291X

VL - 171

SP - 431

EP - 438

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.

Abstract

Keywords

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