Corticotropin-releasing hormone and dexamethasone do not alter secretion of immunoreactive β-endorphin from dissociated fetal hypothalamic cell cultures

Leonard P. Kapcala*, Horng Heng Juang, Ching Feng Weng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

6 Scopus citations

Abstract

Corticotropin-releasing hormone (CRH) and glucocorticoids are major regulators of the hypothalamic-pituitary-adrenal (HPA) axis controlling secretion of β-endorphin and other pro-opiomelanocortin (POMC)-derived peptides from pituitary. Although previous work has shown that CRH stimulates secretion of β-endorphin from adult hypothalamic explants, and that glucocorticoids can inhibit basal and stimulated secretion of POMC-derived peptides from pituitary, the role of glucocorticoids on hyopthalamic β-endorphin secretion is not known. Studies were performed to assess the effects of CRH and dexamethasone, a potent glucocorticoid, on secretion of immunoreactive (IR) β-endorphin from dissociated fetal hypothalamic cell cultures. CRH (10-9-10-6 M) did not stimulate secretion of IR-β-endorphin from hypothalamic cells which did release IR-β-endorphin upon potassium-induced depolarization. However, CRH did stimulate IR-β-endorphin secretion from fetal hypothalamic explants which were similar to hypothalamic tissue from which dissociated hypothalamic cell cultures were derived. Exposure of cells to dexamethasone (10-6 M) did not inhibit basal nor potassium-stimulated release of IR-β-endorphin. These results indicate that: (1) dissociated fetal hypothalamic cells in culture do not exhibit a functional CRH receptor coupled to stimulation of IR-β-endorphin secretion; (2) exposure of hypothalamic cells to dexamethasone does not inhibit basal nor depolarization-induced release of IR-β-endorphin; and (3) dissociated fetal hypothalamic cells may have limited utility in elucidating specific regulatory relationships because of in vitro conditions and/or cytoarchitectural relationships.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBrain Research
Volume532
Issue number1-2
DOIs
StatePublished - 05 11 1990
Externally publishedYes

Keywords

  • Cell culture
  • Corticotropin-releasing hormone
  • Dexamethasone
  • Glucocorticoid
  • Hypothalamus
  • Regulation
  • Secretion
  • β-Endorphin

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