Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Jyh Lyh Juang; Tsan Chi Chen; Shih Sheng Jiang; Chao A. Hsiung; Wei Chen Chen; Guang Wu Chen; Shiang Ming Lin; Jih Hui Lin; Shu Chun Chiu; Yiu Kay Lai

doi:10.1038/labinvest.3700136

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Jyh Lyh Juang^*, Tsan Chi Chen, Shih Sheng Jiang, Chao A. Hsiung, Wei Chen Chen, Guang Wu Chen, Shiang Ming Lin, Jih Hui Lin, Shu Chun Chiu, Yiu Kay Lai

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

21 Scopus citations

Abstract

An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.

Original language	English
Pages (from-to)	1085-1091
Number of pages	7
Journal	Laboratory Investigation
Volume	84
Issue number	9
DOIs	https://doi.org/10.1038/labinvest.3700136
State	Published - 09 2004
Externally published	Yes

Keywords

Coronavirus
Gene chip
Multiplex RT-PCR
SARS
Semiquantitation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/labinvest.3700136

Cite this

@article{358ecc5655de422daacc0327e6327510,

title = "Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus",

abstract = "An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.",

keywords = "Coronavirus, Gene chip, Multiplex RT-PCR, SARS, Semiquantitation",

author = "Juang, \{Jyh Lyh\} and Chen, \{Tsan Chi\} and Jiang, \{Shih Sheng\} and Hsiung, \{Chao A.\} and Chen, \{Wei Chen\} and Chen, \{Guang Wu\} and Lin, \{Shiang Ming\} and Lin, \{Jih Hui\} and Chiu, \{Shu Chun\} and Lai, \{Yiu Kay\}",

year = "2004",

month = sep,

doi = "10.1038/labinvest.3700136",

language = "英语",

volume = "84",

pages = "1085--1091",

journal = "Laboratory Investigation",

issn = "0023-6837",

publisher = "Elsevier B.V.",

number = "9",

}

TY - JOUR

T1 - Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

AU - Juang, Jyh Lyh

AU - Chen, Tsan Chi

AU - Jiang, Shih Sheng

AU - Hsiung, Chao A.

AU - Chen, Wei Chen

AU - Chen, Guang Wu

AU - Lin, Shiang Ming

AU - Lin, Jih Hui

AU - Chiu, Shu Chun

AU - Lai, Yiu Kay

PY - 2004/9

Y1 - 2004/9

N2 - An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.

AB - An early and accurate diagnostic assay for severe acute respiratory syndrome (SARS) is crucial for infection control. However, most of the diagnostic methods available today, such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), require a second detection method for confirmation because they detect a single sequence region of the SARS-coronavirus (SARS-CoV). For sensitive and accurate early diagnosis, we report a novel assay system combining multiplex RT-PCR and a diagnostic gene chip to detect multiple virus-specific genomic sequences of SARS-CoV. With 53 clinical specimens, we successfully demonstrate that this technique offers not only a high-accuracy diagnosis for early SARS infection but also a semiquantitative assay.

KW - Coronavirus

KW - Gene chip

KW - Multiplex RT-PCR

KW - SARS

KW - Semiquantitation

UR - https://www.scopus.com/pages/publications/4344669286

U2 - 10.1038/labinvest.3700136

DO - 10.1038/labinvest.3700136

M3 - 文章

C2 - 15195120

AN - SCOPUS:4344669286

SN - 0023-6837

VL - 84

SP - 1085

EP - 1091

JO - Laboratory Investigation

JF - Laboratory Investigation

IS - 9

ER -

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this