Abstract
A human cell line selected for cisplatin resistance (CPR) was irradiated with UV light and showed cross-resistance to UV light. Applying a modified chloramphenicol acetyltransferase assay, we observed that CPR cells acquired enhanced host cell reactivation of a transfected plasmid carrying UV damage. Gel mobility shift analysis indicated that two nuclear factors that recognize UV-modified DNA were overexpressed in CPR cells. In addition, factors that bind UV-modified DNA were independent from the factors that bind cisplatin-modified DNA. The significance of the identified binding factors, possibly DNA repair enzymes, is discussed.
| Original language | American English |
|---|---|
| Pages (from-to) | 2075-2080 |
| Journal | Molecular and Cellular Biology |
| Volume | 11 |
| Issue number | 4 |
| State | Published - 1991 |
Keywords
- Binding, Competitive
- Cell Line
- Cisplatin/pharmacology
- DNA Repair
- DNA/drug effects
- DNA/metabolism
- DNA/radiation effects
- Dose-Response Relationship, Radiation
- Drug Resistance/genetics
- Gene Expression
- HeLa Cells
- Humans
- Plasmids
- Transfection
- Ultraviolet Rays