Crude extract of Salvia miltiorrhiza and salvianolic acid B enhance in vitro angiogenesis in murine SVR endothelial cell line

Ing Shiow Lay, Jen Hwey Chiu*, Ming Shi Shiao, Wing Yiu Lui, Chew Wun Wu

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

128 Scopus citations

Abstract

Salvia miltiorrhiza (SM) has been used clinically in Asian countries to improve the microcirculation in the human body. Although a pure compound extracted from SM, salvianolic acid B (Sal B), has been reported to be effective against fibrosis and ischemia-reperfusion injury, possibly through its anti-lipid peroxidation action, the effect of SM on angiogenesis remains unclear. It is our interest to investigate the role of SM on the regulation of the angiogenic process. By using the SVR endothelial cell line as an in vitro system, the effects of Sal B on the gene expression of vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, VEGF-R2 were evaluated by morphology, differentiation assay, reverse-transcribed polymerase chain reaction (RT-PCR) and Western blot analysis. The results showed that both the crude extract of SM and the pure compound Sal B had enhancing effects on cell growth and differentiation. The gene expression of matrix metalloproteinase-2 (MMP-2) was up-regulated after Sal B treatment for 2 h, while VEGF and VEGF-R2 gene expression were up-regulated 40 min after Sal B treatment. We conclude that the crude extract of SM and Sal B enhance angiogenic processes on SVR cells through upregulation of VEGF and VEGF receptors genes.

Original languageEnglish
Pages (from-to)26-32
Number of pages7
JournalPlanta Medica
Volume69
Issue number1
DOIs
StatePublished - 01 01 2003
Externally publishedYes

Keywords

  • Angiogenesis
  • Endothelial cells
  • Lamiaceae
  • Salvia miltiorrhiza
  • Salvianolic acid B
  • VEGF

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