Cyclosporine reduces basolateral, but not apical, nitric oxide secretion in medullary thick ascending limb cells

M. S. Wu*, M. Bens, H. M. Yu, A. Vandewalle

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

Cyclosporine (CsA) reduces nitric oxide (NO) production in medullary thick ascending limb (mTAL) cells. We postulated that CsA affected NO secretion in a vectorial manner in polarized renal epithelial cells. The experiments were performed in a model of mTAL subcultured cells. The expression of iNOS in mTAL cells was confirmed by RT-PCR. The cells were grown on a non-permeable filter. Nitrite was measured by the modified Griess method. Transepithelial resistance was measured to ensure the integrity of the tight junction. CsA (100 ng/ml) reduced NO production by 22% in mTAL cells. The inhibitory effect was limited to the basolateral side (control: 165 ± 11; plus CsA: 93 ± 17 nM/106 cells, P < 0.001) without affecting apical NO secretion. The transepithelial resistance through the epithelial monolayer remained unchanged in CsA-treated cells. CsA reduced basolateral NO secretion without affecting apical secretion. The results suggest that CsA might affect intrarenal hemodynamics at the peritubular level.

Original languageEnglish
Pages (from-to)S321-S323
JournalTransplant International
Volume13
Issue numberSUPPL. 1
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Cyclosporine
  • Nitric oxide
  • Thick ascending limb cells

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