Cytotoxic effect of s-petasin and iso-s-petasin on the proliferation of human prostate cancer cells

Zhi Hong Wang, Hui Wen Hsu, Jou Chun Chou, Ching Han Yu, Da Tian Bau, Guei Jane Wang, Chih Yang Huang, Paulus S. Wang, Shyi Wu Wang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

8 Scopus citations

Abstract

Background: Petasin (Petasides hybridus) is a perennial shrub that is found in Europe as well as parts of Asia and North America and is being used to treat hypertension, tumors and asthma. In a previous study, we reported that petasin possesses biological effects including inhibition of testosterone production and the release of corticosterone from rat zona fasciculata-reticularis cells, and anti-proliferative effect on human T24 bladder carcinoma cells.

Materials and Methods: In the present study, we assessed the effects of S-petasin and iso-S-petasin on the growth and proliferation of two hormone-independent DU145 and PC3 and one hormone-dependent LNCaP prostate cancer cell line at concentrations of 10-7-10-5 mol/l. The cell proliferation index, cell number index, expression of caspases and apoptosis-associated proteins and cell morphology were measured.

Results: S-Petasin and iso-Spetasin reduced the viable cell number and increased the numbers of apoptotic cells in the tested cell lines in a dosedependent manner. Western blot analysis revealed that Spetasin and iso-S-petasin reduced the protein levels of procaspase 3, 8, and 9 and cleaved poly(ADP-ribose) polymerase (PARP) in all tested prostate cancer cell lines, and reduced that of procaspase 7 in LNCaP and PC3 cells. At the same time, S-petasin and iso-S-petasin increased mitochondrial membrane permeability and cytochrome c release from mitochondria to the cytosol via reducing the ratio of BCL2/BAX in DU145 and PC3 cells, and upregulating the levels of p53 in DU145 cells but downregulating it in PC3 cells.

Conclusion: These results indicate that S-petasin and iso-S-petasin induce apoptosis via the activation of mitochondria-related pathways in prostate cancer cells, suggesting S-petasin and iso-S-petasin could be potential anticancer agents.

Original languageEnglish
Pages (from-to)191-200
Number of pages10
JournalAnticancer Research
Volume35
Issue number1
StatePublished - 01 01 2015

Keywords

  • Anti-proliferative
  • Apoptosis
  • Petasin
  • Prostate cancer

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