Daphne genkwa Sieb. et Zucc. water-soluble extracts act on enterovirus 71 by inhibiting viral entry

Chia Wen Chang; Yan Lii Leu; Jim Tong Horng

doi:10.3390/v4040539

Daphne genkwa Sieb. et Zucc. water-soluble extracts act on enterovirus 71 by inhibiting viral entry

Chia Wen Chang, Yan Lii Leu, Jim Tong Horng^*

^*Corresponding author for this work

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

25 Scopus citations

Abstract

Dried flowers of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae) are a Chinese herbal medicine used as an abortifacient with purgative, diuretic and anti-inflammatory activities. However, the activity of this medicine against enteroviral infections has not been investigated. The water-extract of dried buds of D. genkwa Sieb. et Zucc. (DGFW) was examined against various strains of enterovirus 71 (EV71) by neutralization assay, and its initial mode of action was characterized by time-of-addition assay followed by attachment and penetration assays. Pretreatment of DGFW with virus abolished viral replication, indicating that DGFW inhibits EV71 by targeting the virus. GFW exerts its anti-EV71 effects by inhibiting viral entry without producing cytotoxic side effects and thus provides a potential agent for antiviral chemotherapeutics.

Original language	English
Pages (from-to)	539-556
Number of pages	18
Journal	Viruses
Volume	4
Issue number	4
DOIs	https://doi.org/10.3390/v4040539
State	Published - 04 2012

Keywords

Attachment assay
Cytopathic effect
Daphne genkwa Sieb. et Zucc.
Enterovirus 71
Penetration assay
Yuanhua

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Daphne genkwa Sieb. et Zucc. water-soluble extracts act on enterovirus 71 by inhibiting viral entry",

abstract = "Dried flowers of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae) are a Chinese herbal medicine used as an abortifacient with purgative, diuretic and anti-inflammatory activities. However, the activity of this medicine against enteroviral infections has not been investigated. The water-extract of dried buds of D. genkwa Sieb. et Zucc. (DGFW) was examined against various strains of enterovirus 71 (EV71) by neutralization assay, and its initial mode of action was characterized by time-of-addition assay followed by attachment and penetration assays. Pretreatment of DGFW with virus abolished viral replication, indicating that DGFW inhibits EV71 by targeting the virus. GFW exerts its anti-EV71 effects by inhibiting viral entry without producing cytotoxic side effects and thus provides a potential agent for antiviral chemotherapeutics.",

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author = "Chang, {Chia Wen} and Leu, {Yan Lii} and Horng, {Jim Tong}",

year = "2012",

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doi = "10.3390/v4040539",

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AU - Leu, Yan Lii

AU - Horng, Jim Tong

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N2 - Dried flowers of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae) are a Chinese herbal medicine used as an abortifacient with purgative, diuretic and anti-inflammatory activities. However, the activity of this medicine against enteroviral infections has not been investigated. The water-extract of dried buds of D. genkwa Sieb. et Zucc. (DGFW) was examined against various strains of enterovirus 71 (EV71) by neutralization assay, and its initial mode of action was characterized by time-of-addition assay followed by attachment and penetration assays. Pretreatment of DGFW with virus abolished viral replication, indicating that DGFW inhibits EV71 by targeting the virus. GFW exerts its anti-EV71 effects by inhibiting viral entry without producing cytotoxic side effects and thus provides a potential agent for antiviral chemotherapeutics.

AB - Dried flowers of Daphne genkwa Sieb. et Zucc. (Thymelaeaceae) are a Chinese herbal medicine used as an abortifacient with purgative, diuretic and anti-inflammatory activities. However, the activity of this medicine against enteroviral infections has not been investigated. The water-extract of dried buds of D. genkwa Sieb. et Zucc. (DGFW) was examined against various strains of enterovirus 71 (EV71) by neutralization assay, and its initial mode of action was characterized by time-of-addition assay followed by attachment and penetration assays. Pretreatment of DGFW with virus abolished viral replication, indicating that DGFW inhibits EV71 by targeting the virus. GFW exerts its anti-EV71 effects by inhibiting viral entry without producing cytotoxic side effects and thus provides a potential agent for antiviral chemotherapeutics.

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KW - Cytopathic effect

KW - Daphne genkwa Sieb. et Zucc.

KW - Enterovirus 71

KW - Penetration assay

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Daphne genkwa Sieb. et Zucc. water-soluble extracts act on enterovirus 71 by inhibiting viral entry

Abstract

Keywords

UN SDGs

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