Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma

Angel Chao, Chia Lung Tsai, Pei Chi Wei, Swei Hsueh, An Shine Chao, Chin Jung Wang, Chi Neu Tsai, Yun Shien Lee*, Tzu Hao Wang, Chyong Huey Lai

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

63 Scopus citations

Abstract

We compared microRNA profiles between choriocarcinoma and non-cancerous trophoblasts, and revealed that miR-199b was underexpressed in choriocarcinoma. By computational prediction and microarray studies, SET (protein phosphatase 2A inhibitor) was shown to be one of the target genes regulated by miR-199b. Ectopic expression of miR-199b inhibited endogenous SET protein levels and the activity of the luciferase reporter containing the 3′-UTR of SET. Further comparisons of formalin-fixed paraffin-embedded human choriocarcinoma, mole, and non-cancer trophoblast tissues confirmed the initial findings of low miR-199b expression and SET upregulation in choriocarcinomas, suggesting that microRNA-dysregulated SET protein may account for the rapid growth seen with choriocarcinomas.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalCancer Letters
Volume291
Issue number1
DOIs
StatePublished - 01 05 2010

Keywords

  • Choriocarcinoma
  • Gestation
  • MicroRNA
  • Mole
  • Placenta

Fingerprint

Dive into the research topics of 'Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibitor) in human choriocarcinoma'. Together they form a unique fingerprint.

Cite this