Deguelin Impairs Cell Adhesion, Migration and Invasion of Human Lung Cancer Cells through the NF-ΰ B Signaling Pathways

Yung Ting Hsiao, Ming Jen Fan, An Cheng Huang, Jin Cherng Lien, Jen Jyh Lin, Jaw Chyun Chen, Te Chun Hsia, Rick Sai Chuen Wu, Jing Gung Chung*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

28 Scopus citations

Abstract

Deguelin, a rotenoid, is isolated from a natural plant species, and has biological activities including antitumor function. In the present study, we investigated the effect of deguelin on the cell adhesion, migration and invasion of NCI-H292 human lung cancer cells in vitro. Cell viability was analyzed by using flow cytometer. Cell adhesion was determined by using the cell-matrix adhesion assay. Wound healing assay was used to examine cell migration. Cell migration and invasion were investigated using a Boyden chamber assay. The protein expression was measured by Western blotting and confocal laser microscopy. The electrophoretic mobility shift assay was used to measure NF-ΰ B p65 binding to DNA.We selected the concentrations of deguelin at 0, 0.5, 1.0, 1.5, 2.0 and 2.5μM and we found that those concentrations of deguelin did not induce significant cytotoxic effects on NCI-H292 cells. Thus, we selected those concentrations of deguelin for metastasis assay. We found that deguelin inhibited cell adhesion, migration and invasion in dose-dependent manners that was assayed by wound healing and transwell methods, respectively. Deguelin decreased the expression of MMP-2/-9, SOS 1, Rho A, p-AKT (Thr308), p-ERK1/2, p-p38, p-JNK, NF-ΰ B (p65) and uPA in NCI-H292 cells. Deguelin suppressed the expression of PI3K, SOS 1, NF-ΰ B (p65), but did not significantly affect PKC and Ras in the nuclei of NCI-H292 cells that were confirmed by confocal laser microscopy. We suggest that deguelin may be used as a novel anticancer metastasis of lung cancer in the future.

Original languageEnglish
Pages (from-to)209-229
Number of pages21
JournalAmerican Journal of Chinese Medicine
Volume46
Issue number1
DOIs
StatePublished - 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 World Scientific Publishing Company.

Keywords

  • Adhesion
  • Deguelin
  • Invasion
  • Migration
  • NCI-H292 Cells
  • NF-ΰ B

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