Dehydroepiandrosterone sulfate (DHEAS) suppresses P2X purinoceptor-coupled responses in PC12 cells

Pei Shan Liu; Hsi Lung Hsieh; Chih Ming Lin

doi:10.1016/S0197-0186(01)00027-4

Dehydroepiandrosterone sulfate (DHEAS) suppresses P2X purinoceptor-coupled responses in PC12 cells

Pei Shan Liu^*, Hsi Lung Hsieh, Chih Ming Lin

^*Corresponding author for this work

Soochow University Taiwan

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

Some steroids rapidly alter neuronal excitability through interaction with neurotransmitter-gated ion channels in addition to their well-known genomic effects via intracellular steroid receptors. Such effects were found in GABA receptor, nicotinic receptors, yet not investigated in P2X purinoceptors. In this study, the effects of dehydroepiandrosterone sulfate on the P2 purinoceptor was investigated. Results show that dehydroepiandrosterone sulfate acutely inhibits P2X purinoceptor functions in PC12 cells. Dehydroepiandrosterone sulfate suppressed ATP-induced cytosolic free calcium concentration ([Ca²⁺]_i) rise, cytosolic free sodium concentration ([Na⁺]_i) rise, and dopamine secretion in the presence of external calcium, but had no effect on ATP-induced [Ca²⁺]_i rise in the absence of external calcium or on UTP-induced [Ca²⁺]_i rise in the absence or presence of external calcium. Our data show that dehydroepiandrosterone sulfate exerted its effect on P2X, but not on the P2Y purinoceptors found in PC12 cells. Estradiol and estrone have similar effects on P2X purinoceptor, but dehydroepiandrosterone and progesterone do not.

Original language	English
Pages (from-to)	193-198
Number of pages	6
Journal	Neurochemistry International
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/S0197-0186(01)00027-4
State	Published - 2001
Externally published	Yes

Keywords

Calcium signal
DHEAS
P2 purinoceptor
PC12 cell
Secretion
Steroid

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10.1016/S0197-0186(01)00027-4

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title = "Dehydroepiandrosterone sulfate (DHEAS) suppresses P2X purinoceptor-coupled responses in PC12 cells",

abstract = "Some steroids rapidly alter neuronal excitability through interaction with neurotransmitter-gated ion channels in addition to their well-known genomic effects via intracellular steroid receptors. Such effects were found in GABA receptor, nicotinic receptors, yet not investigated in P2X purinoceptors. In this study, the effects of dehydroepiandrosterone sulfate on the P2 purinoceptor was investigated. Results show that dehydroepiandrosterone sulfate acutely inhibits P2X purinoceptor functions in PC12 cells. Dehydroepiandrosterone sulfate suppressed ATP-induced cytosolic free calcium concentration ([Ca2+]i) rise, cytosolic free sodium concentration ([Na+]i) rise, and dopamine secretion in the presence of external calcium, but had no effect on ATP-induced [Ca2+]i rise in the absence of external calcium or on UTP-induced [Ca2+]i rise in the absence or presence of external calcium. Our data show that dehydroepiandrosterone sulfate exerted its effect on P2X, but not on the P2Y purinoceptors found in PC12 cells. Estradiol and estrone have similar effects on P2X purinoceptor, but dehydroepiandrosterone and progesterone do not.",

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AU - Hsieh, Hsi Lung

AU - Lin, Chih Ming

PY - 2001

Y1 - 2001

N2 - Some steroids rapidly alter neuronal excitability through interaction with neurotransmitter-gated ion channels in addition to their well-known genomic effects via intracellular steroid receptors. Such effects were found in GABA receptor, nicotinic receptors, yet not investigated in P2X purinoceptors. In this study, the effects of dehydroepiandrosterone sulfate on the P2 purinoceptor was investigated. Results show that dehydroepiandrosterone sulfate acutely inhibits P2X purinoceptor functions in PC12 cells. Dehydroepiandrosterone sulfate suppressed ATP-induced cytosolic free calcium concentration ([Ca2+]i) rise, cytosolic free sodium concentration ([Na+]i) rise, and dopamine secretion in the presence of external calcium, but had no effect on ATP-induced [Ca2+]i rise in the absence of external calcium or on UTP-induced [Ca2+]i rise in the absence or presence of external calcium. Our data show that dehydroepiandrosterone sulfate exerted its effect on P2X, but not on the P2Y purinoceptors found in PC12 cells. Estradiol and estrone have similar effects on P2X purinoceptor, but dehydroepiandrosterone and progesterone do not.

AB - Some steroids rapidly alter neuronal excitability through interaction with neurotransmitter-gated ion channels in addition to their well-known genomic effects via intracellular steroid receptors. Such effects were found in GABA receptor, nicotinic receptors, yet not investigated in P2X purinoceptors. In this study, the effects of dehydroepiandrosterone sulfate on the P2 purinoceptor was investigated. Results show that dehydroepiandrosterone sulfate acutely inhibits P2X purinoceptor functions in PC12 cells. Dehydroepiandrosterone sulfate suppressed ATP-induced cytosolic free calcium concentration ([Ca2+]i) rise, cytosolic free sodium concentration ([Na+]i) rise, and dopamine secretion in the presence of external calcium, but had no effect on ATP-induced [Ca2+]i rise in the absence of external calcium or on UTP-induced [Ca2+]i rise in the absence or presence of external calcium. Our data show that dehydroepiandrosterone sulfate exerted its effect on P2X, but not on the P2Y purinoceptors found in PC12 cells. Estradiol and estrone have similar effects on P2X purinoceptor, but dehydroepiandrosterone and progesterone do not.

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Dehydroepiandrosterone sulfate (DHEAS) suppresses P2X purinoceptor-coupled responses in PC12 cells

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