Dehydroepiandrosterone sulfate inhibition of catecholamine secretion from bovine adrenal chromaffin cells

Pei Shan Liu*, Meng Kai Lin, Hsi Lung Hsieh

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

27 Scopus citations

Abstract

Dehydroepiandrosterone sulfate (DHEAS) dose-dependently inhibited [3H]norepinephrine (NE) secretion and the corresponding [Ca2+](i) rise induced by the nicotinic receptor agonist 1,1-dimethyl-4-phenylpoperazimium (DMPP) in bovine chromaffin cells. DHEAS at 10 μM, the physiological concentration in human serum, significantly inhibited both the release of [3H]NE and the rise of [Ca2+](i) induced by DMPP in chromaffin cells. DHEAS also inhibited the [3H]NE release induced by the Na+ channel activator veratridine. However, DHEAS did not affected either the [3H]NE release, or the corresponding [Ca2+](i) rise induced by high K+. Moreover, DHEAS suppressed the [Na+](i) rise induced by either DMPP or high K+ as monitored by the fluorescence 340/380 ratio of SBFI loaded chromaffin cells. Our results suggest that the inhibitory effects of DHEAS on secretion mainly occur at nicotinic receptors as well as at the voltage-dependent Na+ channels.

Original languageEnglish
Pages (from-to)181-184
Number of pages4
JournalNeuroscience Letters
Volume204
Issue number3
DOIs
StatePublished - 09 02 1996
Externally publishedYes

Keywords

  • Bovine adrenal chromaffin cells
  • Ca concentration
  • Cytosolic
  • Cytosolic Na concentration
  • Dehydroepiandrosterone sulfate
  • Nicotinic receptor
  • [H]Norepinephrine secretion

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