Dehydroepiandrosterone sulfate inhibition of catecholamine secretion from bovine adrenal chromaffin cells

Pei Shan Liu; Meng Kai Lin; Hsi Lung Hsieh

doi:10.1016/0304-3940(96)12350-8

Dehydroepiandrosterone sulfate inhibition of catecholamine secretion from bovine adrenal chromaffin cells

Pei Shan Liu^*
, Meng Kai Lin
, Hsi Lung Hsieh

^*Corresponding author for this work

Soochow University Taiwan

Research output: Contribution to journal › Journal Article › peer-review

27 Scopus citations

Abstract

Dehydroepiandrosterone sulfate (DHEAS) dose-dependently inhibited [³H]norepinephrine (NE) secretion and the corresponding [Ca²⁺](i) rise induced by the nicotinic receptor agonist 1,1-dimethyl-4-phenylpoperazimium (DMPP) in bovine chromaffin cells. DHEAS at 10 μM, the physiological concentration in human serum, significantly inhibited both the release of [³H]NE and the rise of [Ca²⁺](i) induced by DMPP in chromaffin cells. DHEAS also inhibited the [³H]NE release induced by the Na⁺ channel activator veratridine. However, DHEAS did not affected either the [³H]NE release, or the corresponding [Ca²⁺](i) rise induced by high K⁺. Moreover, DHEAS suppressed the [Na⁺](i) rise induced by either DMPP or high K⁺ as monitored by the fluorescence 340/380 ratio of SBFI loaded chromaffin cells. Our results suggest that the inhibitory effects of DHEAS on secretion mainly occur at nicotinic receptors as well as at the voltage-dependent Na⁺ channels.

Original language	English
Pages (from-to)	181-184
Number of pages	4
Journal	Neuroscience Letters
Volume	204
Issue number	3
DOIs	https://doi.org/10.1016/0304-3940(96)12350-8
State	Published - 09 02 1996
Externally published	Yes

Keywords

Bovine adrenal chromaffin cells
Ca concentration
Cytosolic
Cytosolic Na concentration
Dehydroepiandrosterone sulfate
Nicotinic receptor
[H]Norepinephrine secretion

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10.1016/0304-3940(96)12350-8

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title = "Dehydroepiandrosterone sulfate inhibition of catecholamine secretion from bovine adrenal chromaffin cells",

abstract = "Dehydroepiandrosterone sulfate (DHEAS) dose-dependently inhibited [3H]norepinephrine (NE) secretion and the corresponding [Ca2+](i) rise induced by the nicotinic receptor agonist 1,1-dimethyl-4-phenylpoperazimium (DMPP) in bovine chromaffin cells. DHEAS at 10 μM, the physiological concentration in human serum, significantly inhibited both the release of [3H]NE and the rise of [Ca2+](i) induced by DMPP in chromaffin cells. DHEAS also inhibited the [3H]NE release induced by the Na+ channel activator veratridine. However, DHEAS did not affected either the [3H]NE release, or the corresponding [Ca2+](i) rise induced by high K+. Moreover, DHEAS suppressed the [Na+](i) rise induced by either DMPP or high K+ as monitored by the fluorescence 340/380 ratio of SBFI loaded chromaffin cells. Our results suggest that the inhibitory effects of DHEAS on secretion mainly occur at nicotinic receptors as well as at the voltage-dependent Na+ channels.",

keywords = "Bovine adrenal chromaffin cells, Ca concentration, Cytosolic, Cytosolic Na concentration, Dehydroepiandrosterone sulfate, Nicotinic receptor, [H]Norepinephrine secretion",

author = "Liu, \{Pei Shan\} and Lin, \{Meng Kai\} and Hsieh, \{Hsi Lung\}",

year = "1996",

month = feb,

day = "9",

doi = "10.1016/0304-3940(96)12350-8",

language = "英语",

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AU - Liu, Pei Shan

AU - Lin, Meng Kai

AU - Hsieh, Hsi Lung

PY - 1996/2/9

Y1 - 1996/2/9

N2 - Dehydroepiandrosterone sulfate (DHEAS) dose-dependently inhibited [3H]norepinephrine (NE) secretion and the corresponding [Ca2+](i) rise induced by the nicotinic receptor agonist 1,1-dimethyl-4-phenylpoperazimium (DMPP) in bovine chromaffin cells. DHEAS at 10 μM, the physiological concentration in human serum, significantly inhibited both the release of [3H]NE and the rise of [Ca2+](i) induced by DMPP in chromaffin cells. DHEAS also inhibited the [3H]NE release induced by the Na+ channel activator veratridine. However, DHEAS did not affected either the [3H]NE release, or the corresponding [Ca2+](i) rise induced by high K+. Moreover, DHEAS suppressed the [Na+](i) rise induced by either DMPP or high K+ as monitored by the fluorescence 340/380 ratio of SBFI loaded chromaffin cells. Our results suggest that the inhibitory effects of DHEAS on secretion mainly occur at nicotinic receptors as well as at the voltage-dependent Na+ channels.

AB - Dehydroepiandrosterone sulfate (DHEAS) dose-dependently inhibited [3H]norepinephrine (NE) secretion and the corresponding [Ca2+](i) rise induced by the nicotinic receptor agonist 1,1-dimethyl-4-phenylpoperazimium (DMPP) in bovine chromaffin cells. DHEAS at 10 μM, the physiological concentration in human serum, significantly inhibited both the release of [3H]NE and the rise of [Ca2+](i) induced by DMPP in chromaffin cells. DHEAS also inhibited the [3H]NE release induced by the Na+ channel activator veratridine. However, DHEAS did not affected either the [3H]NE release, or the corresponding [Ca2+](i) rise induced by high K+. Moreover, DHEAS suppressed the [Na+](i) rise induced by either DMPP or high K+ as monitored by the fluorescence 340/380 ratio of SBFI loaded chromaffin cells. Our results suggest that the inhibitory effects of DHEAS on secretion mainly occur at nicotinic receptors as well as at the voltage-dependent Na+ channels.

KW - Bovine adrenal chromaffin cells

KW - Ca concentration

KW - Cytosolic

KW - Cytosolic Na concentration

KW - Dehydroepiandrosterone sulfate

KW - Nicotinic receptor

KW - [H]Norepinephrine secretion

UR - https://www.scopus.com/pages/publications/0030049058

U2 - 10.1016/0304-3940(96)12350-8

DO - 10.1016/0304-3940(96)12350-8

M3 - 文章

C2 - 8938260

AN - SCOPUS:0030049058

SN - 0304-3940

VL - 204

SP - 181

EP - 184

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Dehydroepiandrosterone sulfate inhibition of catecholamine secretion from bovine adrenal chromaffin cells

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