Detection and partial sequence analysis of Helicobacter pylori DNA in the bile samples

Tin Tsan Lin, Chau Ting Yeh*, Cheng Shyong Wu, Yun Fan Liaw

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

83 Scopus citations


The existence of helicobacter pylori in the biliary tract was investigated. Seven bile samples were included in this study. Among them, six bile samples were collected by percutaneous transhepatic cholangiodrainage and the other by needle aspiration during cholecystectomy. Using nested PCR with two sets of primers homologous to the urease A gene, Helicobacter pylori DNA was detected. Three samples, one from a patient with advanced gastric cancer involving the pancreatic head and two from patients with pancreatic head tumor, were found to be positive for Helicobacter pylori DNA. On the other hand, three samples from patients with cholangiocarcinoma and one from a patient with chronic cholecystitis were all negative. To further verify the specificity of our PCR analysis, partial sequences of the PCR products from the three positive samples were analyzed by direct sequencing. Several silent mutations and a missense mutation (A AA to A GA; Lys-164 to Arg-164) were identified in the urease A gene. We conclude that Helicobacter pylori DNA can be easily detected in the bile samples. The possibility of asymptomatic cholangitis caused by this organism requires further investigation.

Original languageEnglish
Pages (from-to)2214-2219
Number of pages6
JournalDigestive Diseases and Sciences
Issue number10
StatePublished - 10 1995
Externally publishedYes


  • Helicobacter pylori
  • bile samples
  • polymerase chain reaction
  • sequence


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