Detection of blood-brain barrier disruption by contrast-enhanced high frequency ultrasound image: Small animals study

Ching Hsiang Fan*, Chih Kuang Yeh, Po Hong Hsu, Hao Li Liu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Contrast-enhanced magnetic resonance imaging was used to detect the increase of BBB permeability induced by high intensity focus ultrasound (HIFU) with ultrasound contrast agents (UCAs). In this study, contrast-enhanced high-frequency ultrasound imaging was used to detect the enhancement of BBB permeability in small animal experiments. The higher temporal resolution of high-frequency ultrasound imaging provides a potential to monitor the UCAs leakage into the surrounding brain parenchyma. Sprague-Dawley rats were performed craniotomy before the HIFU sonication. One 1.5 MHz HIFU was delivered to the left hemisphere brain with 2.45 MPa pressure. Prior to sonication, UCAs was injected to enhance BBB permeability. Control group rats were sonicated without UCAs injection. Before and after sonications, the rats were individually injected UCAs and immediately a sequence of B-mode images were acquired at 7 fps. Note that the UCAs act as imaging indicators at this moment. We monitored the BBB disruption 6 hours in total and repeated the above mentioned process every hour. The BBB disruptions were verified by means of Evans blue extravasation. To map contrast replenishment after UCAs injection, the echo power in each voxel was summed over the time direction. The results show the power intensity in experiment group is higher than the control group ones. In experiment group, the power intensity in left hemisphere brain is higher than the right side and the power intensity decreases over time. In addition, the spatial map of power consists with the Evans blue histology results. The conclusion is the contrast-enhanced high-frequency ultrasound imaging is feasible to detect the enhancement of BBB permeability.

Original languageEnglish
Title of host publication2009 IEEE International Ultrasonics Symposium and Short Courses, IUS 2009
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1788-1791
Number of pages4
ISBN (Print)9781424443895
DOIs
StatePublished - 2009
Event2009 IEEE International Ultrasonics Symposium, IUS 2009 - Rome, Italy
Duration: 20 09 200923 09 2009

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117

Conference

Conference2009 IEEE International Ultrasonics Symposium, IUS 2009
Country/TerritoryItaly
CityRome
Period20/09/0923/09/09

Keywords

  • Blood-brain barrier
  • High frequency ultrasound
  • Ultrasonic contrast agent

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