Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors

Hsu Chao Hao, Hwan You Chang, Tsung Pao Wang, Da Jeng Yao*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

14 Scopus citations

Abstract

Techniques to separate cells are widely applied in immunology. The technique to separate a specific antigen on a microfluidic platform involves the use of a shear horizontal surface-acoustic-wave (SH-SAW) sensor. With specific antibodies conjugated onto the surface of the SH-SAW sensors, this technique can serve to identify specific cells in bodily fluids. Jurkat cells, used as a target in this work, provide a model of cells in small abundance (1:1000) for isolation and purification with the ultimate goal of targeting even more dilute cells. T cells were separated from a mixed-cell medium on a chip (Jurkat cells/K562 cells, 1/1000). A novel microchamber was developed to capture cells during the purification, which required a large biosample. Cell detection was demonstrated through the performance of genetic identification on the chip.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalJournal of Laboratory Automation
Volume18
Issue number1
DOIs
StatePublished - 02 2013
Externally publishedYes

Keywords

  • Antibodies
  • Antigen specific
  • Microchamber
  • Shear horizontal surface acoustic wave (SH-SAW)
  • T cells

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