Detection of prostate-specific antigen with a paired surface plasma wave biosensor

Li Chen Su; Ran Chou Chen; Ying Chang Li; Ying Feng Chang; Yi Jang Lee; Cheng Chung Lee; Chien Chou

doi:10.1021/ac100071h

Detection of prostate-specific antigen with a paired surface plasma wave biosensor

Li Chen Su, Ran Chou Chen, Ying Chang Li, Ying Feng Chang, Yi Jang Lee, Cheng Chung Lee, Chien Chou^*

^*Corresponding author for this work

Research Center for Emerging Viral Infections

Research output: Contribution to journal › Journal Article › peer-review

36 Scopus citations

Abstract

In this study, we demonstrated that an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) is capable of real-time detection of prostate-specific antigen (PSA) in diluted human serum without labeling. Experimentally, the detection limit of PSPWB was 8.4 × 10^-9 refractive index unit (RIU) and the PSPWB could measure PSA in a phosphate buffered saline solution from 10 fg/mL (∼300 aM) to 100 pg/mL (∼3 pM) successfully, with demonstration of a linear relationship between PSA concentrations and surface plasmon resonance (SPR) signals. Therefore, results were obtained over a wide dynamic range 5 orders of magnitude for analyte concentration. In addition, the PSPWB successfully detected PSA in diluted human serum as well. These experimental results indicate that the PSPWB is capable of detection with high sensitivity over a wide range by using SPR-based biosensors and has a capability of detecting biological analytes in clinical sample without complicated operating procedures.

Original language	English
Pages (from-to)	3714-3718
Number of pages	5
Journal	Analytical Chemistry
Volume	82
Issue number	9
DOIs	https://doi.org/10.1021/ac100071h
State	Published - 01 05 2010

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10.1021/ac100071h

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title = "Detection of prostate-specific antigen with a paired surface plasma wave biosensor",

abstract = "In this study, we demonstrated that an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) is capable of real-time detection of prostate-specific antigen (PSA) in diluted human serum without labeling. Experimentally, the detection limit of PSPWB was 8.4 × 10-9 refractive index unit (RIU) and the PSPWB could measure PSA in a phosphate buffered saline solution from 10 fg/mL (∼300 aM) to 100 pg/mL (∼3 pM) successfully, with demonstration of a linear relationship between PSA concentrations and surface plasmon resonance (SPR) signals. Therefore, results were obtained over a wide dynamic range 5 orders of magnitude for analyte concentration. In addition, the PSPWB successfully detected PSA in diluted human serum as well. These experimental results indicate that the PSPWB is capable of detection with high sensitivity over a wide range by using SPR-based biosensors and has a capability of detecting biological analytes in clinical sample without complicated operating procedures.",

author = "Su, {Li Chen} and Chen, {Ran Chou} and Li, {Ying Chang} and Chang, {Ying Feng} and Lee, {Yi Jang} and Lee, {Cheng Chung} and Chien Chou",

year = "2010",

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doi = "10.1021/ac100071h",

language = "英语",

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T1 - Detection of prostate-specific antigen with a paired surface plasma wave biosensor

AU - Su, Li Chen

AU - Chen, Ran Chou

AU - Li, Ying Chang

AU - Chang, Ying Feng

AU - Lee, Yi Jang

AU - Lee, Cheng Chung

AU - Chou, Chien

PY - 2010/5/1

Y1 - 2010/5/1

N2 - In this study, we demonstrated that an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) is capable of real-time detection of prostate-specific antigen (PSA) in diluted human serum without labeling. Experimentally, the detection limit of PSPWB was 8.4 × 10-9 refractive index unit (RIU) and the PSPWB could measure PSA in a phosphate buffered saline solution from 10 fg/mL (∼300 aM) to 100 pg/mL (∼3 pM) successfully, with demonstration of a linear relationship between PSA concentrations and surface plasmon resonance (SPR) signals. Therefore, results were obtained over a wide dynamic range 5 orders of magnitude for analyte concentration. In addition, the PSPWB successfully detected PSA in diluted human serum as well. These experimental results indicate that the PSPWB is capable of detection with high sensitivity over a wide range by using SPR-based biosensors and has a capability of detecting biological analytes in clinical sample without complicated operating procedures.

AB - In this study, we demonstrated that an amplitude-sensitive paired surface plasma wave biosensor (PSPWB) is capable of real-time detection of prostate-specific antigen (PSA) in diluted human serum without labeling. Experimentally, the detection limit of PSPWB was 8.4 × 10-9 refractive index unit (RIU) and the PSPWB could measure PSA in a phosphate buffered saline solution from 10 fg/mL (∼300 aM) to 100 pg/mL (∼3 pM) successfully, with demonstration of a linear relationship between PSA concentrations and surface plasmon resonance (SPR) signals. Therefore, results were obtained over a wide dynamic range 5 orders of magnitude for analyte concentration. In addition, the PSPWB successfully detected PSA in diluted human serum as well. These experimental results indicate that the PSPWB is capable of detection with high sensitivity over a wide range by using SPR-based biosensors and has a capability of detecting biological analytes in clinical sample without complicated operating procedures.

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JO - Analytical Chemistry

JF - Analytical Chemistry

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Detection of prostate-specific antigen with a paired surface plasma wave biosensor

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