Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Shih Chung Wei; Tsung Liang Chuang; Hui Hsin Lu; Chia Chen Chang; Da Shin Wang; Kung Bin Sung; Chii Wann Lin

doi:10.1109/IEMBS.2011.6090087

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

Shih Chung Wei, Tsung Liang Chuang, Hui Hsin Lu, Chia Chen Chang, Da Shin Wang, Kung Bin Sung^*, Chii Wann Lin

^*Corresponding author for this work

National Taiwan University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400500 nm spots by using two-photon excitation fluorescence microscopy.

Original language	English
Title of host publication	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
Pages	490-493
Number of pages	4
DOIs	https://doi.org/10.1109/IEMBS.2011.6090087
State	Published - 2011
Externally published	Yes
Event	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States Duration: 30 08 2011 → 03 09 2011

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1557-170X

Conference

Conference	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
Country/Territory	United States
City	Boston, MA
Period	30/08/11 → 03/09/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/IEMBS.2011.6090087

Cite this

Wei, S. C., Chuang, T. L., Lu, H. H., Chang, C. C., Wang, D. S., Sung, K. B., & Lin, C. W. (2011). Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 (pp. 490-493). Article 6090087 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/IEMBS.2011.6090087

Wei, Shih Chung ; Chuang, Tsung Liang ; Lu, Hui Hsin et al. / Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. pp. 490-493 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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title = "Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy",

abstract = "Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400500 nm spots by using two-photon excitation fluorescence microscopy.",

author = "Wei, {Shih Chung} and Chuang, {Tsung Liang} and Lu, {Hui Hsin} and Chang, {Chia Chen} and Wang, {Da Shin} and Sung, {Kung Bin} and Lin, {Chii Wann}",

year = "2011",

doi = "10.1109/IEMBS.2011.6090087",

language = "英语",

isbn = "9781424441211",

series = "Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS",

pages = "490--493",

booktitle = "33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011",

note = "33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 ; Conference date: 30-08-2011 Through 03-09-2011",

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Wei, SC, Chuang, TL, Lu, HH, Chang, CC, Wang, DS, Sung, KB & Lin, CW 2011, Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy. in 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011., 6090087, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 490-493, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011, Boston, MA, United States, 30/08/11. https://doi.org/10.1109/IEMBS.2011.6090087

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy. / Wei, Shih Chung; Chuang, Tsung Liang; Lu, Hui Hsin et al.
33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 490-493 6090087 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Wang, Da Shin

AU - Sung, Kung Bin

AU - Lin, Chii Wann

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AB - Tip-enhanced fluorescence of localized DNA replication by loop-mediated isothermal amplification (LAMP) is a potential way to observe real-time biological reaction confined in nanometer scale. We successfully coated Bst polymerase on the apex (100 nm) of an atomic force microscope (AFM) tip and performed localized LAMP reaction of hepatitis B virus (HBV). By using this tip-based reaction, the replicated HBV DNA can be directly imaged to be 400500 nm spots by using two-photon excitation fluorescence microscopy.

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Wei SC, Chuang TL, Lu HH, Chang CC, Wang DS, Sung KB et al. Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 490-493. 6090087. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/IEMBS.2011.6090087

Detection of tip-enhanced fluorescence from loop-mediated isothermal amplification of hepatitis B virus by two-photon microscopy

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