Determination of Catecholamines in Single Adrenal Medullary Cells by Capillary Electrophoresis and Laser-Induced Native Fluorescence

Huan Tsung Chang; Edward S. Yeung

doi:10.1021/ac00102a010

Determination of Catecholamines in Single Adrenal Medullary Cells by Capillary Electrophoresis and Laser-Induced Native Fluorescence

Huan Tsung Chang^*
, Edward S. Yeung

^*Corresponding author for this work

Ames Laboratory

Research output: Contribution to journal › Journal Article › peer-review

141 Scopus citations

Abstract

The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.

Original language	English
Pages (from-to)	1079-1083
Number of pages	5
Journal	Analytical Chemistry
Volume	67
Issue number	6
DOIs	https://doi.org/10.1021/ac00102a010
State	Published - 1995
Externally published	Yes

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title = "Determination of Catecholamines in Single Adrenal Medullary Cells by Capillary Electrophoresis and Laser-Induced Native Fluorescence",

abstract = "The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.",

author = "Chang, \{Huan Tsung\} and Yeung, \{Edward S.\}",

year = "1995",

doi = "10.1021/ac00102a010",

language = "英语",

volume = "67",

pages = "1079--1083",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Determination of Catecholamines in Single Adrenal Medullary Cells by Capillary Electrophoresis and Laser-Induced Native Fluorescence

AU - Chang, Huan Tsung

AU - Yeung, Edward S.

PY - 1995

Y1 - 1995

N2 - The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.

AB - The present study demonstrates that native fluorescence detection combined with capillary electrophoresis separation at low pH provides high sensitivity (down to nanomolar), high resolution, high speed, and low interference for the analysis of catecholamines. Further, this method has been employed successfully for the measurement of the amounts of epinephrine and norepinephrine in individual bovine adrenal medullary cells. Application of this method to the study of neurochemistry is promising.

UR - https://www.scopus.com/pages/publications/0029644301

U2 - 10.1021/ac00102a010

DO - 10.1021/ac00102a010

M3 - 文章

C2 - 7717523

AN - SCOPUS:0029644301

SN - 0003-2700

VL - 67

SP - 1079

EP - 1083

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 6

ER -

Determination of Catecholamines in Single Adrenal Medullary Cells by Capillary Electrophoresis and Laser-Induced Native Fluorescence

Abstract

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