Development of a Sampling Collection Device with Diagnostic Procedures

Jhih Yan Cheng, Mow Jung Feng, Chia Chi Wu, Jane Wang, Ting Chang Chang*, Chao Min Cheng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

7 Scopus citations


Cervicovaginal fluid plays an important role in the detection of many female genital diseases, but the lack of suitable collection devices in the market severely challenges test success rate. Appropriate clinical sampling devices for cervicovaginal fluid collection would help physicians detect diseases and disease states more rapidly, efficiently, and accurately. The objective of this study was to develop a readily usable sampling collection device that would eliminate macromolecular interference and accurately provide specimens for further studies. This study was designed to develop an effective device to collect cervicovaginal fluid from women with symptoms of endometrial lesions, women appearing in the clinic for a routine Papanicolaou smear, and/or women seeking a routine gynecologic checkup. Paper-based assay, ELISA, and qNano were used to provide accurate diagnoses. A total of 103 patients successfully used the developed device to collect cervicovaginal fluid. Some of the collected specimens were used to detect glycogen, lactate, and pH for determining pathogen infection. Other specimen samples were tested for the presence of female genital cancer by comparing interleukin 6 concentration and microvesicle concentration. We proposed a noninvasive screening test for the diagnosis of female genital diseases using a dual-material collection device. The outer, nonwoven fabric portion of this device was designed to filter macromolecules, and the inner cotton portion was designed to absorb cervicovaginal fluid.

Original languageEnglish
Pages (from-to)7591-7596
Number of pages6
JournalAnalytical Chemistry
Issue number15
StatePublished - 02 08 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.


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