Development of a specific system for targeting protein to metallophilic macrophages

Philip R. Taylor, Susanne Zamze, Richard J. Stillion, Simon Y.C. Wong, Siamon Gordon, Luisa Martinez-Pomares*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

39 Scopus citations


The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve mice, these CRL+ cells, metallophilic macrophages (Mφ) in spleen and subcapsular sinus Mφ in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL+ Mφ. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mφ in an immune response in vivo.

Original languageEnglish
Pages (from-to)1963-1968
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number7
StatePublished - 17 02 2004
Externally publishedYes


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