TY - JOUR
T1 - Development of a specific system for targeting protein to metallophilic macrophages
AU - Taylor, Philip R.
AU - Zamze, Susanne
AU - Stillion, Richard J.
AU - Wong, Simon Y.C.
AU - Gordon, Siamon
AU - Martinez-Pomares, Luisa
PY - 2004/2/17
Y1 - 2004/2/17
N2 - The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve mice, these CRL+ cells, metallophilic macrophages (Mφ) in spleen and subcapsular sinus Mφ in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL+ Mφ. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mφ in an immune response in vivo.
AB - The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve mice, these CRL+ cells, metallophilic macrophages (Mφ) in spleen and subcapsular sinus Mφ in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL+ Mφ. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mφ in an immune response in vivo.
UR - http://www.scopus.com/inward/record.url?scp=1242274410&partnerID=8YFLogxK
U2 - 10.1073/pnas.0308490100
DO - 10.1073/pnas.0308490100
M3 - 文章
C2 - 14764901
AN - SCOPUS:1242274410
SN - 0027-8424
VL - 101
SP - 1963
EP - 1968
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -