Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva

Yang Hung Liang; Chia Chen Chang; Chien Cheng Chen; Yu Chu-Su; Chii Wann Lin

doi:10.1016/j.clinbiochem.2012.09.001

Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva

Yang Hung Liang, Chia Chen Chang, Chien Cheng Chen, Yu Chu-Su, Chii Wann Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

55 Scopus citations

Abstract

Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. Results: The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20. U/mL (the cut-off point in cancer patients is around 4. U/mL). The linear range with the Biacore SPR system was 40-300. U/mL. Conclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.

Original language	English
Pages (from-to)	1689-1693
Number of pages	5
Journal	Clinical Biochemistry
Volume	45
Issue number	18
DOIs	https://doi.org/10.1016/j.clinbiochem.2012.09.001
State	Published - 12 2012
Externally published	Yes

Keywords

Biacore SPR
Gold/zinc oxide
Saliva carbohydrate antigen 15-3
Surface plasmon resonance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clinbiochem.2012.09.001

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title = "Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva",

abstract = "Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. Results: The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20. U/mL (the cut-off point in cancer patients is around 4. U/mL). The linear range with the Biacore SPR system was 40-300. U/mL. Conclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.",

keywords = "Biacore SPR, Gold/zinc oxide, Saliva carbohydrate antigen 15-3, Surface plasmon resonance",

author = "Liang, {Yang Hung} and Chang, {Chia Chen} and Chen, {Chien Cheng} and Yu Chu-Su and Lin, {Chii Wann}",

year = "2012",

month = dec,

doi = "10.1016/j.clinbiochem.2012.09.001",

language = "英语",

volume = "45",

pages = "1689--1693",

journal = "Clinical Biochemistry",

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TY - JOUR

T1 - Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva

AU - Liang, Yang Hung

AU - Chang, Chia Chen

AU - Chen, Chien Cheng

AU - Chu-Su, Yu

AU - Lin, Chii Wann

PY - 2012/12

Y1 - 2012/12

N2 - Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. Results: The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20. U/mL (the cut-off point in cancer patients is around 4. U/mL). The linear range with the Biacore SPR system was 40-300. U/mL. Conclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.

AB - Objective: To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. Design and methods: We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. Results: The linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5-20. U/mL (the cut-off point in cancer patients is around 4. U/mL). The linear range with the Biacore SPR system was 40-300. U/mL. Conclusions: These results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.

KW - Biacore SPR

KW - Gold/zinc oxide

KW - Saliva carbohydrate antigen 15-3

KW - Surface plasmon resonance

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AN - SCOPUS:84870712837

SN - 0009-9120

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JO - Clinical Biochemistry

JF - Clinical Biochemistry

IS - 18

ER -

Development of an Au/ZnO thin film surface plasmon resonance-based biosensor immunoassay for the detection of carbohydrate antigen 15-3 in human saliva

Abstract

Keywords

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