Development of an ELISA for myeloperoxidase on microplate: Normal reference values and effect of temperature on specimen preparation

Pi Yueh Chang, Tsu Lan Wu, Chung Chih Hung, Kuo Chien Tsao, Chien Feng Sun, Lily L. Wu, James T. Wu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

27 Scopus citations

Abstract

Background: Myeloperoxidase (MPO), a leukocyte enzyme, is implicated in both the pathogenesis and the progression of atherosclerosis. Methods: We developed a sandwich ELISA on microplate using commercial antibodies for the measurement of plasma MPO. Results: The in-house ELISA has a sensitivity of 15 ng/ml. Both within-day and between-day imprecision were < 10%. The in-house assay was well correlated with the commercial kit from Oxis (γ = 0.96). We have established normal reference range for MPO for apparently healthy Chinese. No statistical difference was found between males and females and the various age groups. Because the coating antibodies used by two different kits are different in their affinities for MPO, the analysis by the in-house ELISA that was approximately three times that of the Oxis kit when testing the same specimens. We found that it is necessary to keep the heparinized whole blood on ice before centrifugation in order to prevent further release of MPO from the leukocytes at room temperature. For the same reason, serum is not recommended for MPO measurement. We also found that either pooled human plasma or serum containing MPO can be used as calibrators. Conclusions: We believe that this ELISA for MPO is useful to assess risk for inflammation, oxidative stress, nitrosative stress, and for predicting cardiac events.

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalClinica Chimica Acta
Volume373
Issue number1-2
DOIs
StatePublished - 11 2006
Externally publishedYes

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