Development of an enhanced sensitivity method for lateral flow immunoassays: An overview of strategies

Chung An Chen; Chi Hung Liu; Natalie Yi Ju Ho; Sheng Liu; Tsung Ting Tsai

doi:10.4103/tcmj.TCMJ-D-25-00061

Development of an enhanced sensitivity method for lateral flow immunoassays: An overview of strategies

Chung An Chen
, Chi Hung Liu
, Natalie Yi Ju Ho
, Sheng Liu
, Tsung Ting Tsai^*

^*Corresponding author for this work

School of Medicine

Chang Gung Memorial Hospital
Chang Gung University

Research output: Contribution to journal › Review article › peer-review

1 Scopus citations

Abstract

Lateral flow immunoassay (LFIA) is a widely recognized point-of-care diagnostic platform for detecting various analytes. It must meet the criteria of REASSURED, but poor sensitivity and lack of precision in test results remain the main bottlenecks of LFIA. Therefore, signal enhancement methods, such as devices, nanoprobes, or detection modes, can improve this platform. In this paper, many representative studies from the past decade on LFIA sensing platforms will be discussed in detail.

Original language	English
Pages (from-to)	11-20
Number of pages	10
Journal	Tzu Chi Medical Journal
Volume	38
Issue number	1
DOIs	https://doi.org/10.4103/tcmj.TCMJ-D-25-00061
State	Published - 01 01 2026

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Publisher Copyright:
© 2026 Tzu Chi Medical Journal.

Keywords

Lateral flow immunoassay
Point-of-care
Sensing system

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10.4103/tcmj.TCMJ-D-25-00061

Cite this

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title = "Development of an enhanced sensitivity method for lateral flow immunoassays: An overview of strategies",

abstract = "Lateral flow immunoassay (LFIA) is a widely recognized point-of-care diagnostic platform for detecting various analytes. It must meet the criteria of REASSURED, but poor sensitivity and lack of precision in test results remain the main bottlenecks of LFIA. Therefore, signal enhancement methods, such as devices, nanoprobes, or detection modes, can improve this platform. In this paper, many representative studies from the past decade on LFIA sensing platforms will be discussed in detail.",

keywords = "Lateral flow immunoassay, Point-of-care, Sensing system",

author = "Chen, \{Chung An\} and Liu, \{Chi Hung\} and Ho, \{Natalie Yi Ju\} and Sheng Liu and Tsai, \{Tsung Ting\}",

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doi = "10.4103/tcmj.TCMJ-D-25-00061",

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T2 - An overview of strategies

AU - Chen, Chung An

AU - Liu, Chi Hung

AU - Ho, Natalie Yi Ju

AU - Liu, Sheng

AU - Tsai, Tsung Ting

PY - 2026/1/1

Y1 - 2026/1/1

N2 - Lateral flow immunoassay (LFIA) is a widely recognized point-of-care diagnostic platform for detecting various analytes. It must meet the criteria of REASSURED, but poor sensitivity and lack of precision in test results remain the main bottlenecks of LFIA. Therefore, signal enhancement methods, such as devices, nanoprobes, or detection modes, can improve this platform. In this paper, many representative studies from the past decade on LFIA sensing platforms will be discussed in detail.

AB - Lateral flow immunoassay (LFIA) is a widely recognized point-of-care diagnostic platform for detecting various analytes. It must meet the criteria of REASSURED, but poor sensitivity and lack of precision in test results remain the main bottlenecks of LFIA. Therefore, signal enhancement methods, such as devices, nanoprobes, or detection modes, can improve this platform. In this paper, many representative studies from the past decade on LFIA sensing platforms will be discussed in detail.

KW - Lateral flow immunoassay

KW - Point-of-care

KW - Sensing system

UR - https://www.scopus.com/pages/publications/105028259323

U2 - 10.4103/tcmj.TCMJ-D-25-00061

DO - 10.4103/tcmj.TCMJ-D-25-00061

M3 - 文献综述

AN - SCOPUS:105028259323

SN - 1016-3190

VL - 38

SP - 11

EP - 20

JO - Tzu Chi Medical Journal

JF - Tzu Chi Medical Journal

IS - 1

ER -

Development of an enhanced sensitivity method for lateral flow immunoassays: An overview of strategies

Abstract

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Keywords

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