Development of an image profiling method for high- throughput single extracellular vesicle analysis

Li Hsien Ho; Zhi Yang Wei; Yi Chien Chen; Yu He Liu; Chia Hung Chen; Tao Yun Yen; Wei Chun Lan; Yun Hsien Chung; I. Hsuan Chou; Hsien Wen Yao; Peng Wei Hsu; Chen Han Huang; Hsing Ying Lin

doi:10.1117/12.3007897

Development of an image profiling method for high- throughput single extracellular vesicle analysis

Li Hsien Ho, Zhi Yang Wei, Yi Chien Chen, Yu He Liu, Chia Hung Chen, Tao Yun Yen, Wei Chun Lan, Yun Hsien Chung, I. Hsuan Chou, Hsien Wen Yao, Peng Wei Hsu^*, Chen Han Huang^*, Hsing Ying Lin^*

^*Corresponding author for this work

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

5-ALA (5-aminolevulinic acid) administration for positioning tumor tissues in craniotomy is growing since the FDA approval has already passed in June 2017 [1]. This may also open a path to the convenient single extracellular vesicle (EV) analysis in liquid biopsy for tracking and analyzing the tumor-derived EVs with PPIX expression, the middle byproduct upon the oral intake of 5-ALA. 5-ALA-based fluorescence is due to preferential accumulation of the fluorophore protoporphyrin-IX (PpIX) in the malignant glioma tissue. Single EV analysis has become technically feasible and promising in studies of early cancer diagnosis and clinical translational medicine applications [2]. It can minimize the heterogenous issue between EVs once capturing a group of low percentage biomarker-positive EVs. There is a need to scale up and implement an easy-to-use single EV analysis platform to be organized into the routine practices of current research and clinical laboratories to enable early cancer diagnostics. We are combining the 5-ALA dyed EV samples with single EV detection method to establish a more efficient and precise approach for patient’s early cancer diagnosis.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference
Editors	Toyohiko Yatagai
Publisher	SPIE
ISBN (Electronic)	9781510663435
DOIs	https://doi.org/10.1117/12.3007897
State	Published - 2023
Event	Biomedical Imaging and Sensing Conference 2023 - Yokohama, Japan Duration: 17 04 2023 → 21 04 2023

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12608
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biomedical Imaging and Sensing Conference 2023
Country/Territory	Japan
City	Yokohama
Period	17/04/23 → 21/04/23

Bibliographical note

Publisher Copyright:
© 2023 SPIE.

Keywords

5-ALA
PpIX
glioblastoma
imaging analysis
single extracellular vesicle analysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/12.3007897

Cite this

Ho, L. H., Wei, Z. Y., Chen, Y. C., Liu, Y. H., Chen, C. H., Yen, T. Y., Lan, W. C., Chung, Y. H., Chou, I. H., Yao, H. W., Hsu, P. W., Huang, C. H., & Lin, H. Y. (2023). Development of an image profiling method for high- throughput single extracellular vesicle analysis. In T. Yatagai (Ed.), Biomedical Imaging and Sensing Conference Article 126080M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608). SPIE. https://doi.org/10.1117/12.3007897

@inproceedings{6ed872c5a70f4c1482b6911037c33c73,

title = "Development of an image profiling method for high- throughput single extracellular vesicle analysis",

abstract = "5-ALA (5-aminolevulinic acid) administration for positioning tumor tissues in craniotomy is growing since the FDA approval has already passed in June 2017 [1]. This may also open a path to the convenient single extracellular vesicle (EV) analysis in liquid biopsy for tracking and analyzing the tumor-derived EVs with PPIX expression, the middle byproduct upon the oral intake of 5-ALA. 5-ALA-based fluorescence is due to preferential accumulation of the fluorophore protoporphyrin-IX (PpIX) in the malignant glioma tissue. Single EV analysis has become technically feasible and promising in studies of early cancer diagnosis and clinical translational medicine applications [2]. It can minimize the heterogenous issue between EVs once capturing a group of low percentage biomarker-positive EVs. There is a need to scale up and implement an easy-to-use single EV analysis platform to be organized into the routine practices of current research and clinical laboratories to enable early cancer diagnostics. We are combining the 5-ALA dyed EV samples with single EV detection method to establish a more efficient and precise approach for patient{\textquoteright}s early cancer diagnosis.",

keywords = "5-ALA, PpIX, glioblastoma, imaging analysis, single extracellular vesicle analysis",

author = "Ho, \{Li Hsien\} and Wei, \{Zhi Yang\} and Chen, \{Yi Chien\} and Liu, \{Yu He\} and Chen, \{Chia Hung\} and Yen, \{Tao Yun\} and Lan, \{Wei Chun\} and Chung, \{Yun Hsien\} and Chou, \{I. Hsuan\} and Yao, \{Hsien Wen\} and Hsu, \{Peng Wei\} and Huang, \{Chen Han\} and Lin, \{Hsing Ying\}",

note = "Publisher Copyright: {\textcopyright} 2023 SPIE.; Biomedical Imaging and Sensing Conference 2023 ; Conference date: 17-04-2023 Through 21-04-2023",

year = "2023",

doi = "10.1117/12.3007897",

language = "英语",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Toyohiko Yatagai",

booktitle = "Biomedical Imaging and Sensing Conference",

address = "美国",

}

Ho, LH, Wei, ZY, Chen, YC, Liu, YH, Chen, CH, Yen, TY, Lan, WC, Chung, YH, Chou, IH, Yao, HW, Hsu, PW, Huang, CH & Lin, HY 2023, Development of an image profiling method for high- throughput single extracellular vesicle analysis. in T Yatagai (ed.), Biomedical Imaging and Sensing Conference., 126080M, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12608, SPIE, Biomedical Imaging and Sensing Conference 2023, Yokohama, Japan, 17/04/23. https://doi.org/10.1117/12.3007897

Development of an image profiling method for high- throughput single extracellular vesicle analysis. / Ho, Li Hsien; Wei, Zhi Yang; Chen, Yi Chien et al.
Biomedical Imaging and Sensing Conference. ed. / Toyohiko Yatagai. SPIE, 2023. 126080M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development of an image profiling method for high- throughput single extracellular vesicle analysis

AU - Ho, Li Hsien

AU - Wei, Zhi Yang

AU - Chen, Yi Chien

AU - Liu, Yu He

AU - Chen, Chia Hung

AU - Yen, Tao Yun

AU - Lan, Wei Chun

AU - Chung, Yun Hsien

AU - Chou, I. Hsuan

AU - Yao, Hsien Wen

AU - Hsu, Peng Wei

AU - Huang, Chen Han

AU - Lin, Hsing Ying

PY - 2023

Y1 - 2023

N2 - 5-ALA (5-aminolevulinic acid) administration for positioning tumor tissues in craniotomy is growing since the FDA approval has already passed in June 2017 [1]. This may also open a path to the convenient single extracellular vesicle (EV) analysis in liquid biopsy for tracking and analyzing the tumor-derived EVs with PPIX expression, the middle byproduct upon the oral intake of 5-ALA. 5-ALA-based fluorescence is due to preferential accumulation of the fluorophore protoporphyrin-IX (PpIX) in the malignant glioma tissue. Single EV analysis has become technically feasible and promising in studies of early cancer diagnosis and clinical translational medicine applications [2]. It can minimize the heterogenous issue between EVs once capturing a group of low percentage biomarker-positive EVs. There is a need to scale up and implement an easy-to-use single EV analysis platform to be organized into the routine practices of current research and clinical laboratories to enable early cancer diagnostics. We are combining the 5-ALA dyed EV samples with single EV detection method to establish a more efficient and precise approach for patient’s early cancer diagnosis.

AB - 5-ALA (5-aminolevulinic acid) administration for positioning tumor tissues in craniotomy is growing since the FDA approval has already passed in June 2017 [1]. This may also open a path to the convenient single extracellular vesicle (EV) analysis in liquid biopsy for tracking and analyzing the tumor-derived EVs with PPIX expression, the middle byproduct upon the oral intake of 5-ALA. 5-ALA-based fluorescence is due to preferential accumulation of the fluorophore protoporphyrin-IX (PpIX) in the malignant glioma tissue. Single EV analysis has become technically feasible and promising in studies of early cancer diagnosis and clinical translational medicine applications [2]. It can minimize the heterogenous issue between EVs once capturing a group of low percentage biomarker-positive EVs. There is a need to scale up and implement an easy-to-use single EV analysis platform to be organized into the routine practices of current research and clinical laboratories to enable early cancer diagnostics. We are combining the 5-ALA dyed EV samples with single EV detection method to establish a more efficient and precise approach for patient’s early cancer diagnosis.

KW - 5-ALA

KW - PpIX

KW - glioblastoma

KW - imaging analysis

KW - single extracellular vesicle analysis

UR - https://www.scopus.com/pages/publications/85176146034

U2 - 10.1117/12.3007897

DO - 10.1117/12.3007897

M3 - 会议稿件

AN - SCOPUS:85176146034

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Imaging and Sensing Conference

A2 - Yatagai, Toyohiko

PB - SPIE

T2 - Biomedical Imaging and Sensing Conference 2023

Y2 - 17 April 2023 through 21 April 2023

ER -

Development of an image profiling method for high- throughput single extracellular vesicle analysis

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

Access to Document

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