Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression

Yun Wen Tong; Kin Fong Lei

doi:10.1109/NMDC57951.2023.10344016

Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression

Yun Wen Tong^*
, Kin Fong Lei

^*Corresponding author for this work

Master Program in Nano-Electronic Engineering and Design

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Conventional biological techniques for analysis of cellular molecular expression are time-consuming and labor-intensive. In this work, a paper-based microfluidic platform was developed to imitate periosteum-bone tissue microenvironment. This platform serves as an in vitro three-dimensional cell co-culture model to analyze cellular crosstalk and molecular expression of 2 different cells. The developed platform consisted of a paper substrate that was sandwiched between PDPC-hydrogel and ADSC-hydrogel suspensions. The unique design allowed for the transfer of cell secretions through the paper substrate, enabling communication between the cells on either side. Cellular crosstalk was investigated by the protein expression on the paper substrate. Additionally, the osteogenesis potential of PDPCs was examined by analyzing the mRNA expressions of the cells following culture. By utilizing the paper-based microfluidic platform, an in vitro periosteum-bone tissue microenvironment was successfully established. This innovative approach provides a promising method to study cellular crosstalk and molecular expression within a 3D co-culture microenvironment. It holds great potential for advancing the development of effective bone regeneration therapies.

Original language	English
Title of host publication	2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	482-483
Number of pages	2
ISBN (Electronic)	9798350335460
DOIs	https://doi.org/10.1109/NMDC57951.2023.10344016
State	Published - 2023
Event	18th IEEE Nanotechnology Materials and Devices Conference, NMDC 2023 - Paestum, Italy Duration: 22 10 2023 → 25 10 2023

Publication series

Name	2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023

Conference

Conference	18th IEEE Nanotechnology Materials and Devices Conference, NMDC 2023
Country/Territory	Italy
City	Paestum
Period	22/10/23 → 25/10/23

Bibliographical note

Publisher Copyright:
© 2023 IEEE.

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10.1109/NMDC57951.2023.10344016

Cite this

Tong, Y. W., & Lei, K. F. (2023). Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression. In 2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023 (pp. 482-483). (2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NMDC57951.2023.10344016

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title = "Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression",

abstract = "Conventional biological techniques for analysis of cellular molecular expression are time-consuming and labor-intensive. In this work, a paper-based microfluidic platform was developed to imitate periosteum-bone tissue microenvironment. This platform serves as an in vitro three-dimensional cell co-culture model to analyze cellular crosstalk and molecular expression of 2 different cells. The developed platform consisted of a paper substrate that was sandwiched between PDPC-hydrogel and ADSC-hydrogel suspensions. The unique design allowed for the transfer of cell secretions through the paper substrate, enabling communication between the cells on either side. Cellular crosstalk was investigated by the protein expression on the paper substrate. Additionally, the osteogenesis potential of PDPCs was examined by analyzing the mRNA expressions of the cells following culture. By utilizing the paper-based microfluidic platform, an in vitro periosteum-bone tissue microenvironment was successfully established. This innovative approach provides a promising method to study cellular crosstalk and molecular expression within a 3D co-culture microenvironment. It holds great potential for advancing the development of effective bone regeneration therapies.",

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series = "2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023",

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Tong, YW & Lei, KF 2023, Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression. in 2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023. 2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023, Institute of Electrical and Electronics Engineers Inc., pp. 482-483, 18th IEEE Nanotechnology Materials and Devices Conference, NMDC 2023, Paestum, Italy, 22/10/23. https://doi.org/10.1109/NMDC57951.2023.10344016

Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression. / Tong, Yun Wen; Lei, Kin Fong.
2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023. Institute of Electrical and Electronics Engineers Inc., 2023. p. 482-483 (2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Lei, Kin Fong

PY - 2023

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N2 - Conventional biological techniques for analysis of cellular molecular expression are time-consuming and labor-intensive. In this work, a paper-based microfluidic platform was developed to imitate periosteum-bone tissue microenvironment. This platform serves as an in vitro three-dimensional cell co-culture model to analyze cellular crosstalk and molecular expression of 2 different cells. The developed platform consisted of a paper substrate that was sandwiched between PDPC-hydrogel and ADSC-hydrogel suspensions. The unique design allowed for the transfer of cell secretions through the paper substrate, enabling communication between the cells on either side. Cellular crosstalk was investigated by the protein expression on the paper substrate. Additionally, the osteogenesis potential of PDPCs was examined by analyzing the mRNA expressions of the cells following culture. By utilizing the paper-based microfluidic platform, an in vitro periosteum-bone tissue microenvironment was successfully established. This innovative approach provides a promising method to study cellular crosstalk and molecular expression within a 3D co-culture microenvironment. It holds great potential for advancing the development of effective bone regeneration therapies.

AB - Conventional biological techniques for analysis of cellular molecular expression are time-consuming and labor-intensive. In this work, a paper-based microfluidic platform was developed to imitate periosteum-bone tissue microenvironment. This platform serves as an in vitro three-dimensional cell co-culture model to analyze cellular crosstalk and molecular expression of 2 different cells. The developed platform consisted of a paper substrate that was sandwiched between PDPC-hydrogel and ADSC-hydrogel suspensions. The unique design allowed for the transfer of cell secretions through the paper substrate, enabling communication between the cells on either side. Cellular crosstalk was investigated by the protein expression on the paper substrate. Additionally, the osteogenesis potential of PDPCs was examined by analyzing the mRNA expressions of the cells following culture. By utilizing the paper-based microfluidic platform, an in vitro periosteum-bone tissue microenvironment was successfully established. This innovative approach provides a promising method to study cellular crosstalk and molecular expression within a 3D co-culture microenvironment. It holds great potential for advancing the development of effective bone regeneration therapies.

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DO - 10.1109/NMDC57951.2023.10344016

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BT - 2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023

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ER -

Tong YW, Lei KF. Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression. In 2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023. Institute of Electrical and Electronics Engineers Inc. 2023. p. 482-483. (2023 IEEE Nanotechnology Materials and Devices Conference, NMDC 2023). doi: 10.1109/NMDC57951.2023.10344016

Development of Paper-based Microfluidic Platform for Analysis of Cellular Crosstalk and Molecular Expression

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