TY - JOUR
T1 - Development of perfusion-based microbioreactor platform capable of providing tunable dynamic compressive loading to 3-D cell culture construct
T2 - Demonstration study of the effect of compressive stimulations on articular chondrocyte functions
AU - Wu, Min Hsien
AU - Wang, Hsin Yao
AU - Tai, Ching Lung
AU - Chang, Yu Han
AU - Chen, Yan Ming
AU - Huang, Song Bin
AU - Chiu, Tzu Keng
AU - Yang, Tzu Chi
AU - Wang, Shih Siou
PY - 2013
Y1 - 2013
N2 - Mechanical compression plays an important role in modulating cell physiology. To precisely investigate the cellular response to compression, a stable culture condition is required. In this study, we developed a microbioreactor platform capable of providing dynamic compressive loading to the 3-D cell culture constructs under a steady environment. The mechanism of generating compression stimulation to cells is based on the pneumatically-driven deformation of a polydimethylsiloxane (PDMS) membrane, which then exerts compressive loading to the cultured cells through a micropillar. By modulating the magnitude and frequency of the applied pneumatic pressure, the compressive loading can be generated in a tunable manner. In this study, the quantitative relationship between the applied pneumatic pressure and the generated compressive strain on the culture construct was established. Moreover, the effects of compression on the cell viability and the metabolic and biosynthetic activities of articular chondrocytes were investigated. The results disclosed that the dynamic compressive loading (51.3% strain, 1 Hz) might up-regulate the metabolic activity and glycosaminoglycan biosynthesis of articular chondrocytes after 5 day culture. Overall, this study presents a micro cell culture device that is capable of exploring the effects of compressive loading on cell physiology in a precise, high-throughput, low-cost, and user-friendly manner.
AB - Mechanical compression plays an important role in modulating cell physiology. To precisely investigate the cellular response to compression, a stable culture condition is required. In this study, we developed a microbioreactor platform capable of providing dynamic compressive loading to the 3-D cell culture constructs under a steady environment. The mechanism of generating compression stimulation to cells is based on the pneumatically-driven deformation of a polydimethylsiloxane (PDMS) membrane, which then exerts compressive loading to the cultured cells through a micropillar. By modulating the magnitude and frequency of the applied pneumatic pressure, the compressive loading can be generated in a tunable manner. In this study, the quantitative relationship between the applied pneumatic pressure and the generated compressive strain on the culture construct was established. Moreover, the effects of compression on the cell viability and the metabolic and biosynthetic activities of articular chondrocytes were investigated. The results disclosed that the dynamic compressive loading (51.3% strain, 1 Hz) might up-regulate the metabolic activity and glycosaminoglycan biosynthesis of articular chondrocytes after 5 day culture. Overall, this study presents a micro cell culture device that is capable of exploring the effects of compressive loading on cell physiology in a precise, high-throughput, low-cost, and user-friendly manner.
KW - Articular chondrocytes
KW - Compressive loading
KW - Mechanical stimulations
KW - Microbioreactors
KW - Perfusion cell culture
UR - http://www.scopus.com/inward/record.url?scp=84875452642&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2012.09.006
DO - 10.1016/j.snb.2012.09.006
M3 - 文章
AN - SCOPUS:84875452642
SN - 0925-4005
VL - 176
SP - 86
EP - 96
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -