Development of techniques to analyse the formation of HIV provirus in primary human macrophages

M. Collin*, W. James, S. Gordon

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

17 Scopus citations

Abstract

The formation of provirus is an important early event in the life cycle of a retrovirus. We describe a system using PCR for the analysis of this process in primary human macrophages infected in vitro with HIV. We show that products of the first template switch can be detected within 8 h after infection at low multiplicity. The methods described provide a rapid assay of infection with HIV and can be applied in the investigation of a number of aspects of HIV replication in macrophages.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalResearch in Virology
Volume142
Issue number2-3
DOIs
StatePublished - 1991
Externally publishedYes

Keywords

  • Diagnosis
  • Early stage
  • HIV
  • Macrophage
  • PCR
  • Provirus
  • Replication

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