Diagnosis of Mycobacterium tuberculosis using palladium-platinum bimetallic nanoparticles combined with paper-based analytical devices

Cheng Yang Tung, Tsung Ting Tsai, Ping Yeh Chiu, Roman Viter, Arũnas Ramanavičius, Cheng Ju Yu*, Chien Fu Chen*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

2 Scopus citations

Abstract

In this study, we demonstrate that palladium-platinum bimetallic nanoparticles (Pd@Pt NPs) as the nanozyme, combined with a multi-layer paper-based analytical device and DNA hybridization, can successfully detect Mycobacterium tuberculosis. This nanozyme has peroxidase-like properties, which can increase the oxidation rate of the substrate. Compared with horseradish peroxidase, which is widely used in traditional detection, the Michaelis constants of Pd@Pt NPs are fourteen and seventeen times lower than those for 3,3′,5,5′-tetramethylbenzidine and H2O2, respectively. To verify the catalytic efficiency of Pd@Pt NPs, this study will execute molecular diagnosis of Mycobacterium tuberculosis. We chose the IS6110 fragment as the target DNA and divided the complementary sequences into the capture DNA and reporter DNA. They were modified on paper and Pd@Pt NPs, respectively, to detect Mycobacterium tuberculosis on a paper-based analytical device. With the above-mentioned method, we can detect target DNA within 15 minutes with a linear range between 0.75 and 10 nM, and a detection limit of 0.216 nM. These results demonstrate that the proposed platform (a DNA-nanozyme integrated paper-based analytical device, dnPAD) can provide sensitive and on-site infection prognosis in areas with insufficient medical resources.

Original languageEnglish
Pages (from-to)5988-5998
Number of pages11
JournalNanoscale
Volume16
Issue number12
DOIs
StatePublished - 21 03 2024

Bibliographical note

Publisher Copyright:
© 2024 The Royal Society of Chemistry.

Keywords

  • Hydrogen Peroxide/chemistry
  • Platinum/chemistry
  • Palladium/chemistry
  • Mycobacterium tuberculosis
  • Metal Nanoparticles/chemistry
  • DNA
  • Colorimetry

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