TY - JOUR
T1 - Diagnosis of Mycobacterium tuberculosis using palladium-platinum bimetallic nanoparticles combined with paper-based analytical devices
AU - Tung, Cheng Yang
AU - Tsai, Tsung Ting
AU - Chiu, Ping Yeh
AU - Viter, Roman
AU - Ramanavičius, Arũnas
AU - Yu, Cheng Ju
AU - Chen, Chien Fu
N1 - Publisher Copyright:
© 2024 The Royal Society of Chemistry.
PY - 2024/3/21
Y1 - 2024/3/21
N2 - In this study, we demonstrate that palladium-platinum bimetallic nanoparticles (Pd@Pt NPs) as the nanozyme, combined with a multi-layer paper-based analytical device and DNA hybridization, can successfully detect Mycobacterium tuberculosis. This nanozyme has peroxidase-like properties, which can increase the oxidation rate of the substrate. Compared with horseradish peroxidase, which is widely used in traditional detection, the Michaelis constants of Pd@Pt NPs are fourteen and seventeen times lower than those for 3,3′,5,5′-tetramethylbenzidine and H2O2, respectively. To verify the catalytic efficiency of Pd@Pt NPs, this study will execute molecular diagnosis of Mycobacterium tuberculosis. We chose the IS6110 fragment as the target DNA and divided the complementary sequences into the capture DNA and reporter DNA. They were modified on paper and Pd@Pt NPs, respectively, to detect Mycobacterium tuberculosis on a paper-based analytical device. With the above-mentioned method, we can detect target DNA within 15 minutes with a linear range between 0.75 and 10 nM, and a detection limit of 0.216 nM. These results demonstrate that the proposed platform (a DNA-nanozyme integrated paper-based analytical device, dnPAD) can provide sensitive and on-site infection prognosis in areas with insufficient medical resources.
AB - In this study, we demonstrate that palladium-platinum bimetallic nanoparticles (Pd@Pt NPs) as the nanozyme, combined with a multi-layer paper-based analytical device and DNA hybridization, can successfully detect Mycobacterium tuberculosis. This nanozyme has peroxidase-like properties, which can increase the oxidation rate of the substrate. Compared with horseradish peroxidase, which is widely used in traditional detection, the Michaelis constants of Pd@Pt NPs are fourteen and seventeen times lower than those for 3,3′,5,5′-tetramethylbenzidine and H2O2, respectively. To verify the catalytic efficiency of Pd@Pt NPs, this study will execute molecular diagnosis of Mycobacterium tuberculosis. We chose the IS6110 fragment as the target DNA and divided the complementary sequences into the capture DNA and reporter DNA. They were modified on paper and Pd@Pt NPs, respectively, to detect Mycobacterium tuberculosis on a paper-based analytical device. With the above-mentioned method, we can detect target DNA within 15 minutes with a linear range between 0.75 and 10 nM, and a detection limit of 0.216 nM. These results demonstrate that the proposed platform (a DNA-nanozyme integrated paper-based analytical device, dnPAD) can provide sensitive and on-site infection prognosis in areas with insufficient medical resources.
KW - Hydrogen Peroxide/chemistry
KW - Platinum/chemistry
KW - Palladium/chemistry
KW - Mycobacterium tuberculosis
KW - Metal Nanoparticles/chemistry
KW - DNA
KW - Colorimetry
UR - http://www.scopus.com/inward/record.url?scp=85187577305&partnerID=8YFLogxK
U2 - 10.1039/d3nr05508f
DO - 10.1039/d3nr05508f
M3 - 文章
C2 - 38465745
AN - SCOPUS:85187577305
SN - 2040-3364
VL - 16
SP - 5988
EP - 5998
JO - Nanoscale
JF - Nanoscale
IS - 12
ER -