Diagnostic devices for isothermal nucleic acid amplification

Chia Chen Chang, Chien Cheng Chen, Shih Chung Wei, Hui Hsin Lu, Yang Hung Liang, Chii Wann Lin*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

108 Scopus citations


Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.

Original languageEnglish
Pages (from-to)8319-8337
Number of pages19
Issue number6
StatePublished - 06 2012
Externally publishedYes


  • HDA
  • Isothermal nucleic acid amplification
  • LAMP
  • RCA
  • SDA


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