Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins

Albert M. Wu*, June H. Wu, Winifred M. Watkins, Chie Pein Chen, Shuh Chyung Song, Yuen Yuen Chen

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

13 Scopus citations

Abstract

The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a- ) THGP.

Original languageEnglish
Pages (from-to)323-326
Number of pages4
JournalFEBS Letters
Volume429
Issue number3
DOIs
StatePublished - 16 06 1998

Keywords

  • Binding properties of GalNAc specific lectins
  • Native and asialo-Tamm- Horsfall glycoproteins

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