Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins

Albert M. Wu; June H. Wu; Winifred M. Watkins; Chie Pein Chen; Shuh Chyung Song; Yuen Yuen Chen

doi:10.1016/S0014-5793(98)00617-6

Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B₄ agglutinins

Albert M. Wu^*, June H. Wu, Winifred M. Watkins, Chie Pein Chen, Shuh Chyung Song, Yuen Yuen Chen

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

13 Scopus citations

Abstract

The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B₄ (VVA-B₄), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B₄ and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B₄ are useful reagents to differentiate between Sd(a+) and Sd(a- ) THGP.

Original language	English
Pages (from-to)	323-326
Number of pages	4
Journal	FEBS Letters
Volume	429
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(98)00617-6
State	Published - 16 06 1998

Keywords

Binding properties of GalNAc specific lectins
Native and asialo-Tamm- Horsfall glycoproteins

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title = "Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins",

abstract = "The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a- ) THGP.",

keywords = "Binding properties of GalNAc specific lectins, Native and asialo-Tamm- Horsfall glycoproteins",

author = "Wu, {Albert M.} and Wu, {June H.} and Watkins, {Winifred M.} and Chen, {Chie Pein} and Song, {Shuh Chyung} and Chen, {Yuen Yuen}",

year = "1998",

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T1 - Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins

AU - Wu, Albert M.

AU - Wu, June H.

AU - Watkins, Winifred M.

AU - Chen, Chie Pein

AU - Song, Shuh Chyung

AU - Chen, Yuen Yuen

PY - 1998/6/16

Y1 - 1998/6/16

N2 - The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a- ) THGP.

AB - The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc α-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAcβ→ was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a- ) THGP.

KW - Binding properties of GalNAc specific lectins

KW - Native and asialo-Tamm- Horsfall glycoproteins

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U2 - 10.1016/S0014-5793(98)00617-6

DO - 10.1016/S0014-5793(98)00617-6

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ER -

Differential binding of human blood group Sd(a+) and Sd(a-) Tamm- Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B₄ agglutinins

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