Differential expression of cytokine genes in cervical cancer tissues

Chia C. Pao*, Chieh Yu Lin, Ding Shyan Yao, Chih Jen Tseng

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

42 Scopus citations

Abstract

The expression of genes coding for inflammatory cytokine interleukin-1-alpha (IL-1α), IL-6, interferon-γ and tumor necrosis factor-α from 15 normal cervix, 11 cervical intraepithelial neoplasia and 13 cervical cancer tissues was investigated. The cytokine messenger ribonucleic acids were reverse transcribed and amplified in the presence of biotinylated and dinitrophenylated primers. Amplified DNA was then captured onto streptavidin-coated microwell plate and quantitatively measured in a colorimetric reaction using ant-DNP antibodies conjugated to horse radish peroxidase. There is no change of IL-1α, IL-6 and tumor necrosis factor-α gene expression in either cervical intraepithelial neoplasia or cervical cancer tissues. But the transcription of interferon-γ gene is significantly reduced in both cervical intraepithelial neoplasia and cervical cancer tissues as compared to normal cervix. This study demonstrated that reverse transcription and quantitative polymerase chain reaction coupling to colorimetric microwell plate assay is a sensitive and useful method to quantitate multiple cytokine gene expression. Our results also suggest that cervical epithelial cells are capable to express cytokines and that interferon-γ may play a role in the pathogenesis of cervical cancer since its reduced expression may influence inflammation and immunity of the cervical tissues.

Original languageEnglish
Pages (from-to)1146-1151
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number3
DOIs
StatePublished - 25 09 1995

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