Differential proteomics analysis of amniotic fluid in pregnancies of increased nuchal translucency with normal karyotype

Po Jen Cheng, Tzu Hao Wang, Shang Yu Huang, Chuan Chi Kao, Jen Hao Lu, Ching Hwa Hsiao, S. W. Steven Shaw*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

18 Scopus citations

Abstract

To investigate the functional roles of differentially expressed proteins in amniotic fluid supernatant (AFS) from normal karyotype pregnancies with increased nuchal translucency (NT). +Methods: AFS from fetuses with increased NT (>3.4 mm, N = 14) and control (<0.7 mm, N = 14) were analyzed by two-dimensional electrophoresis and in-gel digestion to identify the difference of expressed proteins between both groups. Targeted proteins were confirmed by western blot and ELISA. The roles of biological networks in pathophysiology of NT were determined using MetaCore mapping. +Results: Levels of apolipoprotein-A1 (Entrez Gene ID 335), alpha-1 antitrypsin (SERPINA1, ID 5265) and prolactin (ID 5617) were significantly decreased in AFS of fetuses with increased NT compared with those in controls. According to Gene Ontology terms, biological processes of functional networks were mainly involved in steroid metabolism. On the basis of the database of MetaCore, these proteins are considered as the potential biomarkers of cardiovascular disorders. The prediction of tissue distribution from this network in fetal organs such as liver, skin, and kidney supported that these three proteins may play different roles between two groups. +Conclusion: The combination of using quantitative proteomics and functional network analysis could integrally analyze the pathophysiology of fetuses with increased NT.

Original languageEnglish
Pages (from-to)274-281
Number of pages8
JournalPrenatal Diagnosis
Volume31
Issue number3
DOIs
StatePublished - 03 2011

Keywords

  • Amniotic fluid
  • Nuchal translucency
  • Pregnancy
  • Proteomics

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