Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay

Po Chi Soo; Yu Tze Horng; Po Ren Hsueh; Bin Jon Shen; Jann Yuan Wang; Hui Hsin Tu; Jun Rong Wei; Shang Chen Hsieh; Chien Chung Huang; Hsin Chih Lai

doi:10.1016/j.mimet.2006.01.010

Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay

Po Chi Soo, Yu Tze Horng, Po Ren Hsueh, Bin Jon Shen, Jann Yuan Wang, Hui Hsin Tu, Jun Rong Wei, Shang Chen Hsieh, Chien Chung Huang, Hsin Chih Lai^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

49 Scopus citations

Abstract

The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. The currently commercially available molecular assays which use either IS6110 or 16S rDNA fragment as identification targets are mainly designed for identifying MTBC but not for MTB. Comparative genomic DNA analysis has provided valuable information on regions of difference (RD) present in MTB but not in other members of the MTBC. RD9 region is further suggested to be a potential target for differential identification of MTB from MTBC. In this study, using IS6110 and Rv3618 (belong to RD9) as the specific identification targets for MTBC and MTB, respectively, we developed and tested a multiplex nested PCR-ICT (immuno-chromatography test) assay for simultaneously and directly detecting not only MTBC but also MTB from 1500 clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients' clinical assessments. This assay showed a 95.5% sensitivity, 97.9% specificity, 2.1% false positive rate and 4.5% false negative rate towards detection of MTBC, and a 93.0% sensitivity, 99.8% specificity, 0.2% false positive rate and 7.0% false negative rate for detection of MTB. This detection system shows great potential in clinical application.

Original language	English
Pages (from-to)	440-448
Number of pages	9
Journal	Journal of Microbiological Methods
Volume	66
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2006.01.010
State	Published - 09 2006
Externally published	Yes

Keywords

Immuno-chromatography test (ICT)
Molecular detection
Multiplex nested PCR
Mycobacterium tuberculosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mimet.2006.01.010

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Soo, P. C., Horng, Y. T., Hsueh, P. R., Shen, B. J., Wang, J. Y., Tu, H. H., Wei, J. R., Hsieh, S. C., Huang, C. C., & Lai, H. C. (2006). Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay. Journal of Microbiological Methods, 66(3), 440-448. https://doi.org/10.1016/j.mimet.2006.01.010

@article{f3981f1fae0044fda7b333ed9a0c84db,

title = "Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay",

abstract = "The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. The currently commercially available molecular assays which use either IS6110 or 16S rDNA fragment as identification targets are mainly designed for identifying MTBC but not for MTB. Comparative genomic DNA analysis has provided valuable information on regions of difference (RD) present in MTB but not in other members of the MTBC. RD9 region is further suggested to be a potential target for differential identification of MTB from MTBC. In this study, using IS6110 and Rv3618 (belong to RD9) as the specific identification targets for MTBC and MTB, respectively, we developed and tested a multiplex nested PCR-ICT (immuno-chromatography test) assay for simultaneously and directly detecting not only MTBC but also MTB from 1500 clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients' clinical assessments. This assay showed a 95.5% sensitivity, 97.9% specificity, 2.1% false positive rate and 4.5% false negative rate towards detection of MTBC, and a 93.0% sensitivity, 99.8% specificity, 0.2% false positive rate and 7.0% false negative rate for detection of MTB. This detection system shows great potential in clinical application.",

keywords = "Immuno-chromatography test (ICT), Molecular detection, Multiplex nested PCR, Mycobacterium tuberculosis",

author = "Soo, {Po Chi} and Horng, {Yu Tze} and Hsueh, {Po Ren} and Shen, {Bin Jon} and Wang, {Jann Yuan} and Tu, {Hui Hsin} and Wei, {Jun Rong} and Hsieh, {Shang Chen} and Huang, {Chien Chung} and Lai, {Hsin Chih}",

year = "2006",

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doi = "10.1016/j.mimet.2006.01.010",

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volume = "66",

pages = "440--448",

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Soo, PC, Horng, YT, Hsueh, PR, Shen, BJ, Wang, JY, Tu, HH, Wei, JR, Hsieh, SC, Huang, CC & Lai, HC 2006, 'Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay', Journal of Microbiological Methods, vol. 66, no. 3, pp. 440-448. https://doi.org/10.1016/j.mimet.2006.01.010

TY - JOUR

T1 - Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay

AU - Soo, Po Chi

AU - Horng, Yu Tze

AU - Hsueh, Po Ren

AU - Shen, Bin Jon

AU - Wang, Jann Yuan

AU - Tu, Hui Hsin

AU - Wei, Jun Rong

AU - Hsieh, Shang Chen

AU - Huang, Chien Chung

AU - Lai, Hsin Chih

PY - 2006/9

Y1 - 2006/9

N2 - The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. The currently commercially available molecular assays which use either IS6110 or 16S rDNA fragment as identification targets are mainly designed for identifying MTBC but not for MTB. Comparative genomic DNA analysis has provided valuable information on regions of difference (RD) present in MTB but not in other members of the MTBC. RD9 region is further suggested to be a potential target for differential identification of MTB from MTBC. In this study, using IS6110 and Rv3618 (belong to RD9) as the specific identification targets for MTBC and MTB, respectively, we developed and tested a multiplex nested PCR-ICT (immuno-chromatography test) assay for simultaneously and directly detecting not only MTBC but also MTB from 1500 clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients' clinical assessments. This assay showed a 95.5% sensitivity, 97.9% specificity, 2.1% false positive rate and 4.5% false negative rate towards detection of MTBC, and a 93.0% sensitivity, 99.8% specificity, 0.2% false positive rate and 7.0% false negative rate for detection of MTB. This detection system shows great potential in clinical application.

AB - The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. The currently commercially available molecular assays which use either IS6110 or 16S rDNA fragment as identification targets are mainly designed for identifying MTBC but not for MTB. Comparative genomic DNA analysis has provided valuable information on regions of difference (RD) present in MTB but not in other members of the MTBC. RD9 region is further suggested to be a potential target for differential identification of MTB from MTBC. In this study, using IS6110 and Rv3618 (belong to RD9) as the specific identification targets for MTBC and MTB, respectively, we developed and tested a multiplex nested PCR-ICT (immuno-chromatography test) assay for simultaneously and directly detecting not only MTBC but also MTB from 1500 clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients' clinical assessments. This assay showed a 95.5% sensitivity, 97.9% specificity, 2.1% false positive rate and 4.5% false negative rate towards detection of MTBC, and a 93.0% sensitivity, 99.8% specificity, 0.2% false positive rate and 7.0% false negative rate for detection of MTB. This detection system shows great potential in clinical application.

KW - Immuno-chromatography test (ICT)

KW - Molecular detection

KW - Multiplex nested PCR

KW - Mycobacterium tuberculosis

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U2 - 10.1016/j.mimet.2006.01.010

DO - 10.1016/j.mimet.2006.01.010

M3 - 文章

C2 - 16516314

AN - SCOPUS:33745968720

SN - 0167-7012

VL - 66

SP - 440

EP - 448

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Direct and Simultaneous Identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by Rapid Multiplex nested PCR-ICT assay

Abstract

Keywords

UN SDGs

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