Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

Yi Shan Yang; Karen Smith-McCune; Teresa M. Darragh; Yvonne Lai; Ju Hwa Lin; Ting Chang Chang; Hsiao Yun Guo; Tiea Kesler; Alicia Carter; Philip E. Castle; Shuling Cheng

doi:10.1128/CVI.00388-12

Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

Yi Shan Yang
, Karen Smith-McCune
, Teresa M. Darragh
, Yvonne Lai
, Ju Hwa Lin
, Ting Chang Chang
, Hsiao Yun Guo
, Tiea Kesler
, Alicia Carter
, Philip E. Castle
, Shuling Cheng^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

12 Scopus citations

Abstract

A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

Original language	English
Pages (from-to)	1474-1479
Number of pages	6
Journal	Clinical and Vaccine Immunology
Volume	19
Issue number	9
DOIs	https://doi.org/10.1128/CVI.00388-12
State	Published - 09 2012
Externally published	Yes

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Yang, Y. S., Smith-McCune, K., Darragh, T. M., Lai, Y., Lin, J. H., Chang, T. C., Guo, H. Y., Kesler, T., Carter, A., Castle, P. E., & Cheng, S. (2012). Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples. Clinical and Vaccine Immunology, 19(9), 1474-1479. https://doi.org/10.1128/CVI.00388-12

@article{38e426076fa1479f80154f0faee4ad87,

title = "Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples",

abstract = "A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83\% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72\% tested E6 positive and 92\% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31\% and 47\% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).",

author = "Yang, \{Yi Shan\} and Karen Smith-McCune and Darragh, \{Teresa M.\} and Yvonne Lai and Lin, \{Ju Hwa\} and Chang, \{Ting Chang\} and Guo, \{Hsiao Yun\} and Tiea Kesler and Alicia Carter and Castle, \{Philip E.\} and Shuling Cheng",

year = "2012",

month = sep,

doi = "10.1128/CVI.00388-12",

language = "英语",

volume = "19",

pages = "1474--1479",

journal = "Clinical and Vaccine Immunology",

issn = "1556-6811",

publisher = "American Society for Microbiology",

number = "9",

}

Yang, YS, Smith-McCune, K, Darragh, TM, Lai, Y, Lin, JH, Chang, TC, Guo, HY, Kesler, T, Carter, A, Castle, PE & Cheng, S 2012, 'Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples', Clinical and Vaccine Immunology, vol. 19, no. 9, pp. 1474-1479. https://doi.org/10.1128/CVI.00388-12

TY - JOUR

T1 - Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

AU - Yang, Yi Shan

AU - Smith-McCune, Karen

AU - Darragh, Teresa M.

AU - Lai, Yvonne

AU - Lin, Ju Hwa

AU - Chang, Ting Chang

AU - Guo, Hsiao Yun

AU - Kesler, Tiea

AU - Carter, Alicia

AU - Castle, Philip E.

AU - Cheng, Shuling

PY - 2012/9

Y1 - 2012/9

N2 - A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

AB - A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

UR - https://www.scopus.com/pages/publications/84866004230

U2 - 10.1128/CVI.00388-12

DO - 10.1128/CVI.00388-12

M3 - 文章

C2 - 22815148

AN - SCOPUS:84866004230

SN - 1556-6811

VL - 19

SP - 1474

EP - 1479

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 9

ER -

Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

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