Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth

Pamela Pruski; Gonçalo D.S. Correia; Holly V. Lewis; Katia Capuccini; Paolo Inglese; Denise Chan; Richard G. Brown; Lindsay Kindinger; Yun S. Lee; Ann Smith; Julian Marchesi; Julie A.K. McDonald; Simon Cameron; Kate Alexander-Hardiman; Anna L. David; Sarah J. Stock; Jane E. Norman; Vasso Terzidou; T. G. Teoh; Lynne Sykes; Phillip R. Bennett; Zoltan Takats; David A. MacIntyre

doi:10.1038/s41467-021-26215-w

Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth

Pamela Pruski, Gonçalo D.S. Correia, Holly V. Lewis, Katia Capuccini, Paolo Inglese, Denise Chan, Richard G. Brown, Lindsay Kindinger, Yun S. Lee, Ann Smith, Julian Marchesi, Julie A.K. McDonald, Simon Cameron, Kate Alexander-Hardiman, Anna L. David, Sarah J. Stock, Jane E. Norman, Vasso Terzidou, T. G. Teoh, Lynne SykesPhillip R. Bennett, Zoltan Takats^*, David A. MacIntyre^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

45 Scopus citations

Abstract

The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.

Original language	English
Article number	5967
Journal	Nature Communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-26215-w
State	Published - 01 12 2021
Externally published	Yes

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Publisher Copyright:
© 2021, The Author(s).

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Pruski, P., Correia, G. D. S., Lewis, H. V., Capuccini, K., Inglese, P., Chan, D., Brown, R. G., Kindinger, L., Lee, Y. S., Smith, A., Marchesi, J., McDonald, J. A. K., Cameron, S., Alexander-Hardiman, K., David, A. L., Stock, S. J., Norman, J. E., Terzidou, V., Teoh, T. G., ... MacIntyre, D. A. (2021). Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth. Nature Communications, 12(1), Article 5967. https://doi.org/10.1038/s41467-021-26215-w

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title = "Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth",

abstract = "The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.",

author = "Pamela Pruski and Correia, {Gon{\c c}alo D.S.} and Lewis, {Holly V.} and Katia Capuccini and Paolo Inglese and Denise Chan and Brown, {Richard G.} and Lindsay Kindinger and Lee, {Yun S.} and Ann Smith and Julian Marchesi and McDonald, {Julie A.K.} and Simon Cameron and Kate Alexander-Hardiman and David, {Anna L.} and Stock, {Sarah J.} and Norman, {Jane E.} and Vasso Terzidou and Teoh, {T. G.} and Lynne Sykes and Bennett, {Phillip R.} and Zoltan Takats and MacIntyre, {David A.}",

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year = "2021",

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doi = "10.1038/s41467-021-26215-w",

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Pruski, P, Correia, GDS, Lewis, HV, Capuccini, K, Inglese, P, Chan, D, Brown, RG, Kindinger, L, Lee, YS, Smith, A, Marchesi, J, McDonald, JAK, Cameron, S, Alexander-Hardiman, K, David, AL, Stock, SJ, Norman, JE, Terzidou, V, Teoh, TG, Sykes, L, Bennett, PR, Takats, Z & MacIntyre, DA 2021, 'Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth', Nature Communications, vol. 12, no. 1, 5967. https://doi.org/10.1038/s41467-021-26215-w

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AU - Pruski, Pamela

AU - Correia, Gonçalo D.S.

AU - Lewis, Holly V.

AU - Capuccini, Katia

AU - Inglese, Paolo

AU - Chan, Denise

AU - Brown, Richard G.

AU - Kindinger, Lindsay

AU - Lee, Yun S.

AU - Smith, Ann

AU - Marchesi, Julian

AU - McDonald, Julie A.K.

AU - Cameron, Simon

AU - Alexander-Hardiman, Kate

AU - David, Anna L.

AU - Stock, Sarah J.

AU - Norman, Jane E.

AU - Terzidou, Vasso

AU - Teoh, T. G.

AU - Sykes, Lynne

AU - Bennett, Phillip R.

AU - Takats, Zoltan

AU - MacIntyre, David A.

PY - 2021/12/1

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N2 - The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.

AB - The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.

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SN - 2041-1723

VL - 12

JO - Nature Communications

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Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth

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