Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

Chien Wei Ooi; Beng Ti Tey; Siew Ling Hii; Arbakariya Ariff; Ho Shing Wu; John Chi Wei Lan; Ruey Shin Juang; Siti Mazlina Mustapa Kamal; Tau Chuan Ling

doi:10.1007/s12257-008-0306-y

Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

Chien Wei Ooi, Beng Ti Tey, Siew Ling Hii, Arbakariya Ariff, Ho Shing Wu, John Chi Wei Lan, Ruey Shin Juang, Siti Mazlina Mustapa Kamal, Tau Chuan Ling

Research output: Contribution to journal › Journal Article › peer-review

60 Scopus citations

Abstract

An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei lipase from fermentation broth. The partition behavior of B. pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (V_R), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.

Original language	English
Pages (from-to)	811-818
Number of pages	8
Journal	Biotechnology and Bioprocess Engineering
Volume	14
Issue number	6
DOIs	https://doi.org/10.1007/s12257-008-0306-y
State	Published - 12 2009
Externally published	Yes

Keywords

Aqueous two-phase systems
Lipase
Polyethylene glycol
Purification
Recovery

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10.1007/s12257-008-0306-y

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title = "Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems",

abstract = "An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei lipase from fermentation broth. The partition behavior of B. pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.",

keywords = "Aqueous two-phase systems, Lipase, Polyethylene glycol, Purification, Recovery",

author = "Ooi, {Chien Wei} and Tey, {Beng Ti} and Hii, {Siew Ling} and Arbakariya Ariff and Wu, {Ho Shing} and Lan, {John Chi Wei} and Juang, {Ruey Shin} and Kamal, {Siti Mazlina Mustapa} and Ling, {Tau Chuan}",

year = "2009",

month = dec,

doi = "10.1007/s12257-008-0306-y",

language = "英语",

volume = "14",

pages = "811--818",

journal = "Biotechnology and Bioprocess Engineering",

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T1 - Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

AU - Ooi, Chien Wei

AU - Tey, Beng Ti

AU - Hii, Siew Ling

AU - Ariff, Arbakariya

AU - Wu, Ho Shing

AU - Lan, John Chi Wei

AU - Juang, Ruey Shin

AU - Kamal, Siti Mazlina Mustapa

AU - Ling, Tau Chuan

PY - 2009/12

Y1 - 2009/12

N2 - An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei lipase from fermentation broth. The partition behavior of B. pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.

AB - An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei lipase from fermentation broth. The partition behavior of B. pseudomallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study.

KW - Aqueous two-phase systems

KW - Lipase

KW - Polyethylene glycol

KW - Purification

KW - Recovery

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DO - 10.1007/s12257-008-0306-y

M3 - 文章

AN - SCOPUS:77149155962

SN - 1226-8372

VL - 14

SP - 811

EP - 818

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 6

ER -

Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems

Abstract

Keywords

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