TY - JOUR
T1 - Discontinuous DNA replication in a lig-7 strain of Escherichia coli is not the result of mismatch repair, nucleotide-excision repair, or the base-excision repair of DNA uracil
AU - Wang, Tzu chien V.
AU - Smith, Kendric C.
PY - 1989/12/15
Y1 - 1989/12/15
N2 - After pulse-labeling with 3H-thymidine for 30 s at 42°C, the newly-synthesized DNA from uvrB5 liq-7, uvrB5 liq-7 ung-1 (or ung152), uvrB5 lig-7 mutL218 (or mutS215), and uvrB5 lig-7 ung-1 mutL218 (or mutS215) cells sedimented very slowly in alkaline sucrose gradients. The bulk of these DNA molecules were smaller than 2,000 nucleotides long (i.e., about the size of Okazaki fragments), and none of the 3H-radioactivity was found to sediment as high-molecular-weight DNA. These results indicate that the apparent discontinuous DNA replication observed in lig-7 strains is not the result of mismatch repair, nucleotide-excision repair, or the base-excision repair of DNA uracil.
AB - After pulse-labeling with 3H-thymidine for 30 s at 42°C, the newly-synthesized DNA from uvrB5 liq-7, uvrB5 liq-7 ung-1 (or ung152), uvrB5 lig-7 mutL218 (or mutS215), and uvrB5 lig-7 ung-1 mutL218 (or mutS215) cells sedimented very slowly in alkaline sucrose gradients. The bulk of these DNA molecules were smaller than 2,000 nucleotides long (i.e., about the size of Okazaki fragments), and none of the 3H-radioactivity was found to sediment as high-molecular-weight DNA. These results indicate that the apparent discontinuous DNA replication observed in lig-7 strains is not the result of mismatch repair, nucleotide-excision repair, or the base-excision repair of DNA uracil.
UR - http://www.scopus.com/inward/record.url?scp=0024849301&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(89)80020-8
DO - 10.1016/S0006-291X(89)80020-8
M3 - 文章
C2 - 2688644
AN - SCOPUS:0024849301
SN - 0006-291X
VL - 165
SP - 685
EP - 688
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -