Discovery of tumor markers for gastric cancer by proteomics

Jeng Yih Wu; Chun Chia Cheng; Jaw Yuan Wang; Deng Chyang Wu; Jan Sing Hsieh; Shui Cheng Lee; Wen Ming Wang

doi:10.1371/journal.pone.0084158

Discovery of tumor markers for gastric cancer by proteomics

Jeng Yih Wu, Chun Chia Cheng, Jaw Yuan Wang, Deng Chyang Wu, Jan Sing Hsieh, Shui Cheng Lee, Wen Ming Wang

Research output: Contribution to journal › Journal Article › peer-review

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Abstract

Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered. This study intended to directly discover differential biomarkers from GC tissues by two-dimension differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC). Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG. Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions. This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.

Original language	English
Article number	e84158
Journal	PLoS ONE
Volume	9
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0084158
State	Published - 03 01 2014
Externally published	Yes

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title = "Discovery of tumor markers for gastric cancer by proteomics",

abstract = "Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered. This study intended to directly discover differential biomarkers from GC tissues by two-dimension differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC). Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG. Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions. This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.",

author = "Wu, {Jeng Yih} and Cheng, {Chun Chia} and Wang, {Jaw Yuan} and Wu, {Deng Chyang} and Hsieh, {Jan Sing} and Lee, {Shui Cheng} and Wang, {Wen Ming}",

year = "2014",

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doi = "10.1371/journal.pone.0084158",

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AU - Wu, Jeng Yih

AU - Cheng, Chun Chia

AU - Wang, Jaw Yuan

AU - Wu, Deng Chyang

AU - Hsieh, Jan Sing

AU - Lee, Shui Cheng

AU - Wang, Wen Ming

PY - 2014/1/3

Y1 - 2014/1/3

N2 - Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered. This study intended to directly discover differential biomarkers from GC tissues by two-dimension differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC). Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG. Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions. This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.

AB - Gastric cancer (GC) has a high rate of morbidity and mortality among various cancers worldwide. The development of noninvasive diagnostic methods or technologies for tracking the occurrence of GC is urgent, and searching reliable biomarkers is considered. This study intended to directly discover differential biomarkers from GC tissues by two-dimension differential gel electrophoresis (2D-DIGE), and further validate protein expression by western blotting (WB) and immunohistochemistry (IHC). Pairs of GC tissues (gastric cancer tissues and the adjacent normal tissues) obtained from surgery was investigated for 2D-DIEG. Five proteins wereconfirmed by WB and IHC, including glucose-regulated protein 78 (GRP78), glutathione s-transferase pi (GSTpi), apolipoprotein AI (ApoAI), alpha-1 antitrypsin (A1AT) and gastrokine-1 (GKN-1). Among the results, GRP78, GSTpi and A1ATwere significantlyup-regulated and down-regulated respectively in gastric cancer patients. Moreover, GRP78 and ApoAI were correlated with A1AT for protein expressions. This study presumes these proteins could be candidates of reliable biomarkers for gastric cancer.

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Discovery of tumor markers for gastric cancer by proteomics

Abstract

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