Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor

Iain Fraser*, Derralynn Hughes, Siamon Gordon

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

314 Scopus citations

Abstract

MACROPHAGES interact with other cells and components of the extracellular environment by means of adhesion receptors1,2. Adhesion to artificial substrata in vitro facilitates isolation of macrophages3, and has been used to generate antibodies that inhibit their migration in vivo 4,5. Unlike other cell types, macrophages attach to tissue culture plastic in the absence of divalent cations. Here we use an adhesion assay exploiting this property to isolate a rat monoclonal antibody, 2F8, which totally inhibits divalent cation-independent adhesion of murine macrophages to tissue culture plastic in the presence of fetal calf serum. Immunoprecipitation from macrophages and stably transfected Chinese hamster ovary cells revealed that the antigen recognized by monoclonal 2F8 is identical to murine macrophage scavenger receptor6,7. We propose a novel function for this molecule, previously described as an endocytic receptor, thus providing a mechanism for mononuclear phagocyte recruitment to and retention in ligand-rich tissues such as in atherosclerotic lesions.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalNature
Volume364
Issue number6435
DOIs
StatePublished - 1993
Externally publishedYes

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