Divergent Characteristics of T-Cell Receptor Repertoire Between Essential Hypertension and Aldosterone-Producing Adenoma

Che Mai Chang, Kang Yung Peng, Chieh Kai Chan, Yu Feng Lin, Hung Wei Liao, Jan Gowth Chang, Mai Szu Wu, Vin Cent Wu*, Wei Chiao Chang*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

4 Scopus citations


Aldosterone-producing adenoma (APA) is a benign adrenal tumor that results in persistent hyperaldosteronism. As one major subtype of primary aldosteronism, APA leads to secondary hypertension that is associated with immune dysregulation. However, how the adaptive immune system, particularly the T-cell population, is altered in APA patients remains largely unknown. Here, we performed TCR sequencing to characterize the TCR repertoire between two age-matched groups of patients: one with APA and the other one with essential hypertension (EH). Strikingly, we found a significant reduction of TCR repertoire diversity in the APA group. Analyses on TCR clustering and antigen annotation further showed that the APA group possessed lower diversity in TCR clonotypes with non-common antigen-specific features, compared with the EH group. In addition, our results indicated that the strength of correlation between generation probabilities and frequencies of TCR clonotypes was significantly higher in the APA group than that in the EH group. Finally, we observed that clinical features, including plasma aldosterone level, aldosterone–renin ratio, and blood sodium level, were positively associated with the strength of correlation between generation and abundance of TCR clonotypes in the APA group. Our findings unveiled the correlation between T-cell immune repertoire and APA, suggesting a critical role of such adrenal adenoma in the T-cell immunity of patients with hypertension.

Original languageEnglish
Article number853403
JournalFrontiers in Immunology
StatePublished - 10 05 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
Copyright © 2022 Chang, Peng, Chan, Lin, Liao, Chang, Wu, Wu and Chang.


  • T-cell receptor (TCR)
  • TCR repertoire
  • Taiwan Primary Aldosteronism Investigation (TAIPAI)
  • aldosterone-producing adenoma (APA)
  • high-throughput sequencing
  • hypertension


Dive into the research topics of 'Divergent Characteristics of T-Cell Receptor Repertoire Between Essential Hypertension and Aldosterone-Producing Adenoma'. Together they form a unique fingerprint.

Cite this